ROCK2 Protein, Human (Active, sf9, His)

ROCK2 Protein, Human (Active, sf9, His) is the recombinant human-derived ROCK2, expressed by Sf9 insect cells , with C-8*His labeled tag.

ROCK2 is a protein kinase that serves as a key regulator of the actin cytoskeleton and cell polarity. It modulates smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion, and motility by phosphorylating proteins such as ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, and VIM. Additionally, ROCK2 phosphorylates SORL1 and IRF4 and acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. During myogenic differentiation, it positively regulates the activation of p42/MAPK1-p44/MAPK3 and p90RSK/RPS6KA1. ROCK2 plays a crucial role in the timely initiation of centrosome duplication and inhibits keratinocyte terminal differentiation. It may regulate eyelid closure and ventral body wall formation by organizing actomyosin bundles. In the hippocampus, ROCK2 is critical for regulating spine and synaptic properties and contributes to the circadian rhythm of aortic myofilament Ca²⁺ sensitivity and vascular contractility by modulating myosin light chain phosphorylation.

Measured by its ability to catalyze 0.2 mg/mL S6K synthetic peptide substrate and 25 μM ATP. which can be measured by Luminescence that incubate at ambient temperature for 40 mins. The specific activity is 93.03 nmo/min/mg.

Human

Sf9 insect cells

C-8*His

O75116 (M1-S553)

9475

Partial

MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQIS

Approximately 65 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution in PBS, pH 7.5, 1 mM DTT, 0.05% Brij-35, 10% Glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.