RYBP Protein, Human (sf9, His-GST)
RYBP Protein, a crucial regulatory factor, complexes with metalloproteinases like collagenases, inhibiting their activity by irreversibly binding to catalytic zinc cofactors.This interaction, notably with MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9, highlights RYBP's pivotal role in finely regulating extracellular matrix remodeling and proteolytic processes across diverse physiological contexts.RYBP Protein, Human (sf9, His-GST) is the recombinant human-derived RYBP protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RYBP Protein, a crucial regulatory factor, complexes with metalloproteinases like collagenases, inhibiting their activity by irreversibly binding to catalytic zinc cofactors.This interaction, notably with MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9, highlights RYBP's pivotal role in finely regulating extracellular matrix remodeling and proteolytic processes across diverse physiological contexts.RYBP Protein, Human (sf9, His-GST) is the recombinant human-derived RYBP protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
TIMP-4, a key regulatory protein, forms complexes with metalloproteinases, including collagenases, exerting its inhibitory effect by irreversibly binding to their catalytic zinc cofactors. This interaction is known to specifically impact metalloproteinases such as MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9. Through this binding mechanism, TIMP-4 plays a crucial role in modulating the activity of these metalloproteinases, contributing to the fine-tuned regulation of extracellular matrix remodeling and proteolytic processes in various physiological contexts.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-His;N-GST
-
Accession
Q8N488 (M1-F228)
-
Molecular Construction
-
N-term
-
His-GST
-
RYBP (M1-F228)
Accession # Q8N488 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
RYBP; RING1 And YY1-Binding Protein; RING1 And YY1 Binding Protein; Ring1 Interactor RYBP; YEAF1; DED-Associated Factor; DEDAF; APAP-1; Death Effector Domain-Associated Factor; RING1 And YY1 Binding Protein, Isoform CRA_b; Apoptin-Associating Protein 1; Y
-
AA Sequence
MTMGDKKSPTRPKRQAKPAADEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF
-
Predicted Molecular Mass
52.6 kDa
-
Molecular Weight
Approximately 58 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
