SARS-CoV-2 3CLpro/3C-like protease Protein (sf9, His-Avi)
SARS-CoV-2 3CLpro/3C-like protease Protein (sf9, His-Avi) is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by Sf9 insect cells, with N-His, N-Avi labeled tag.
- Species: Virus
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SARS-CoV-2 3CLpro/3C-like protease Protein (sf9, His-Avi) is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by Sf9 insect cells, with N-His, N-Avi labeled tag.
Background
nsp3 (papain-like protease) is a key functional protein in the viral replicase polyprotein, mainly responsible for the cleavage of the N-terminus of the replicase polyprotein; it participates in the assembly of virus-induced cytoplasmic double-membrane vesicles (DMVs) together with nsp4 (DMVs are necessary for viral replication) and plays a role in its formation and maintenance (DMVs are formed by nsp3 and nsp4, and nsp6 is involved in the connection of the endoplasmic reticulum membrane and the binding to lipid droplets). At the same time, nsp3 inhibits the induction of type I interferon by blocking the phosphorylation, dimerization and nuclear translocation of host IRF3, and can also inhibit NF-κB signaling, thereby antagonizing innate immunity. In addition, it has deubiquitination/de-ISG activity, can process Lys-48 and Lys-63-linked polyubiquitin chains in cellular substrates, preferentially cuts ISG15 from the antiviral protein IFIH1 (MDA5) (has no effect on RIGI), and participates in the host ADP-ribosylation process.
Technical Parameters
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Species Virus
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Source Sf9 insect cells
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Tag N-His;N-Avi
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Accession
P0DTC1-1/YP_009725295.1 (S3264-Q3569)
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Gene ID43740578 [NCBI]
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Molecular Construction
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N-term
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His-Avi
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SARS-CoV-2 3CLpro (S3264-Q3569)
Accession # P0DTC1-1/YP_009725295.1 -
C-term
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Protein Length
Full Length of 3C-like proteinase nsp5 Chain
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Synonyms
3CL Protease; 3CL Proteinase; 3C-like main protease; 3CL-Mpro; COVID-19; M Proteinase; Main Protease; Mpro
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AA Sequence
SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ
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Predicted Molecular Mass
37.1 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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