Serpin E2 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Serpin E2, a serine protease inhibitor, inhibits thrombin, trypsin, and urokinase, with a notable role in promoting neurite extension by specifically inhibiting thrombin.Its ability to bind heparin underscores interactions with glycosaminoglycans.The multifaceted inhibitory functions of Serpin E2 highlight its significance in regulating serine protease activities and potential impact on cellular processes, particularly in neurodevelopment.Serpin E2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin E2 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Serpin E2, a serine protease inhibitor, inhibits thrombin, trypsin, and urokinase, with a notable role in promoting neurite extension by specifically inhibiting thrombin.Its ability to bind heparin underscores interactions with glycosaminoglycans.The multifaceted inhibitory functions of Serpin E2 highlight its significance in regulating serine protease activities and potential impact on cellular processes, particularly in neurodevelopment.Serpin E2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin E2 protein, expressed by HEK293 , with C-10*His labeled tag.
Serpin E2, a serine protease inhibitor, exhibits inhibitory activity against thrombin, trypsin, and urokinase. Notably, it plays a role in promoting neurite extension by specifically inhibiting thrombin, emphasizing its involvement in neurodevelopmental processes. Additionally, Serpin E2 is recognized for its ability to bind heparin, showcasing its interaction with this important glycosaminoglycan. The multifaceted inhibitory functions of Serpin E2 underscore its significance in regulating serine protease activities and its potential impact on cellular processes, particularly in the context of neurodevelopment.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 at room temperature for 30 minutes. The IC50 value is 2.644 nM.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
Q07235 (S20-P397)
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Molecular Construction
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N-term
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Serpin E2 (S20-P397)
Accession # Q07235 -
10*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
SERPINE2; Serpin E2; Prev. PI7; PI-7; PN1; PN-1; GDN; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 2; PNI; Glial-Derived Neurite Promoting Factor; Serpin Peptidase Inhibitor, Clade E (Nexin, Pl
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AA Sequence
SQFNSLSLEELGSNTGIQVFNQIIKSRPHENVVVSPHGIASILGMLQLGADGKTKKQLSTVMRYNVNGVGKVLKKINKAIVSKKNKDIVTVANAVFLRNGFKMEVPFAVRNKDVFQCEVQNVNFQDPASASESINFWVKNETRGMIDNLLSPNLIDGALTRLVLVNAVYFKGLWKSRFQPESTKKRTFVAGDGKSYQVPMLAQLSVFRSGSTRTPNGLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHITTKTIDSWMNTMVPKRMQLVLPKFTAVAQTDLKEPLKALGITEMFEPSKANFTKITRSESLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFSIRHNPTGAILFLGQVNKP
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Molecular Weight
Approximately 50-55 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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