Serpin G1 Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
Serpin G1, also known as C1 inhibitor, controls C1 complex activation by forming a proteolytically inactive complex with C1r or C1s proteases. Their interactions regulate physiological pathways such as complement activation, coagulation, fibrinolysis, and kinin production. Serpin G1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin G1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Serpin G1, also known as C1 inhibitor, controls C1 complex activation by forming a proteolytically inactive complex with C1r or C1s proteases. Their interactions regulate physiological pathways such as complement activation, coagulation, fibrinolysis, and kinin production. Serpin G1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin G1 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
Serpin G1, known as C1-inhibitor, exerts control over the activation of the C1 complex by forming a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. This interaction highlights its potential pivotal role in regulating essential physiological pathways, including complement activation, blood coagulation, fibrinolysis, and kinin generation. Functioning as a highly efficient inhibitor of FXIIa, it also inhibits chymotrypsin and kallikrein. Remarkably, Serpin G1 binds to E. coli stcE, facilitating its localization to cell membranes and thereby safeguarding the bacteria against complement-mediated lysis. Additionally, it engages with MASP1, further contributing to its intricate involvement in diverse cellular processes.
Verified Bioactivity
Measured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is < 15 nM, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (1)
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Journal Impact Factor
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Most Recent
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Food Chem Toxicol
Aristolochic acid I promotes renal tubulointerstitial fibrosis by activating the mannan-binding lectin serine protease 1-complement system. [Abstract]2025 Nov:205:115708. PMID: 40846107
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P05155/NP_000053.2 (N23-A500)
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Molecular Construction
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N-term
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Serpin G1 (N23-A500)
Accession # P05155/NP_000053.2 -
10*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
SERPING1; Serine (Or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1, (Angioedema, Hereditary); Prev. C1NH; Serine/Cysteine Proteinase Inhibitor Clade G Member 1 Splice Variant 2; Plasma Protease C1 Inhibitor; Serine/Cysteine Proteinase I
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AA Sequence
NPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA
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Molecular Weight
Approximately 110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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