SHH Protein, Mouse (HEK293, His)

Sonic hedgehog (SHH) undergoes autoproteolysis in the endoplasmic reticulum to produce ShhN and ShhC. ShhN is covalently linked to cholesterol to form ShhNp, a morphogen critical for developmental patterning. SHH Protein, Mouse (HEK293, His) is the recombinant mouse-derived SHH protein, expressed by HEK293 , with C-His labeled tag. The total length of SHH Protein, Mouse (HEK293, His) is 174 a.a., with molecular weight of ~23 kDa.

The sonic hedgehog protein (SHH) precursor undergoes autoproteolysis and cholesterol transferase activity, resulting in the cleavage of the protein into two parts, ShhN and ShhC. The newly generated ShhN is then covalently attached with a cholesterol moiety at its C-terminal. This process occurs in the endoplasmic reticulum. ShhC is subsequently degraded in the endoplasmic reticulum. The lipidated ShhNp acts as a morphogen and plays a crucial role in various developmental patterning events. It induces ventral cell fate in the neural tube and somites, participates in the anterior-posterior axis patterning of the limb bud, and is essential for axon guidance. SHH binds to the patched (PTCH1) receptor, which, in association with smoothened (SMO), activates the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 3.6-40 μg/mL.

Mouse

HEK293

C-His

Q62226 (C25-G198)

20423  [NCBI]

Approximately 23 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.