Siglec-6 Protein, Human (HEK293, His-Avi)

Siglec-6 Protein, a putative adhesion molecule, facilitates sialic acid-dependent binding to cells, specifically binding to alpha-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Siglec-6 interacts with LEP. Siglec-6 Protein, Human (HEK293, His-Avi) is the recombinant human-derived Siglec-6 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The Siglec-6 Protein is a putative adhesion molecule that functions by mediating sialic acid-dependent binding to cells, specifically binding to alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-6 may be masked by cis interactions with sialic acids on the same cell surface, suggesting a dynamic regulation of its binding properties. In addition to its adhesion role, Siglec-6 interacts with LEP, implying its involvement in cellular interactions and signaling processes. The multifaceted nature of Siglec-6 underscores its potential as a key player in sialic acid-mediated cellular adhesion and communication pathways.

Immobilized Human Siglec-6, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Siglec-6 Antibody, hFc Tag with the EC₅₀ of 3-3.5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-8*His

O43699-3 (Q27-V331)

946  [NCBI]

Partial

QERRFQLEGPESLTVQEGLCVLVPCRLPTTLPASYYGYGYWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRDNAAYFFRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGTPPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHSTNLTCQVTFPGAGVTMERTIQLNVSSFKILQNTSSLPVLEGQALRLLCDADGNPPAHLSWFQGFPALNATPISNTGVLELPQVGSAEEGDFTCRAQHPLGSLQISLSLFVHWKPEGRAGGV

Approximately 55-70 kDa, based on Bis-Tris PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing Bis-Tris PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.