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  4. Siglec-7 Protein, Human (HEK293, His)

Siglec-7 Protein, Human (HEK293, His)

Cat. No.: HY-P71311
COA Handling Instructions

Siglec-7 protein is a putative adhesion molecule that mediates sialic acid-dependent cell binding with preference for α-2,3- and α-2,6-linked sialic acids.It interacts with disialoganglioside and may be masked by cis interactions.Siglec-7 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-7 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $185 In-stock
50 μg $520 In-stock
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Description

Siglec-7 protein is a putative adhesion molecule that mediates sialic acid-dependent cell binding with preference for α-2,3- and α-2,6-linked sialic acids.It interacts with disialoganglioside and may be masked by cis interactions.Siglec-7 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-7 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Siglec-7 protein, identified as a putative adhesion molecule, serves as a mediator of sialic-acid dependent cellular binding. It exhibits a preference for binding to alpha-2,3- and alpha-2,6-linked sialic acid, along with interactions with disialogangliosides such as disialogalactosyl globoside, disialyl lactotetraosylceramide, and disialyl GalNAc lactotetraoslylceramide. Notably, the sialic acid recognition site of Siglec-7 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it functions as an inhibitory receptor, inducing tyrosine phosphorylation upon ligand binding and recruiting cytoplasmic phosphatases through their SH2 domains, thereby blocking signal transduction and inhibiting natural killer cell cytotoxicity. Siglec-7 may also play a role in hemopoiesis, inhibiting the differentiation of CD34+ cell precursors towards the myelomonocytic cell lineage and impeding the proliferation of leukemic myeloid cells in vitro. Additionally, it interacts with PTPN6/SHP-1 upon phosphorylation.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9Y286 (Q19-L353)

Gene ID
Molecular Construction
N-term
Siglec-7 (Q19-L353)
Accession # Q9Y286
6*His
C-term
Synonyms
Sialic acid-binding Ig-like lectin 7; AIRM-1; D-siglec; CD328; SIGLEC7
AA Sequence

QKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLL

Molecular Weight

50-70 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Siglec-7 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-7 Protein, Human (HEK293, His)
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HY-P71311
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