Siglec-7 Protein, Human (HEK293, His)

Siglec-7 protein is a putative adhesion molecule that mediates sialic acid-dependent cell binding with preference for α-2,3- and α-2,6-linked sialic acids. It interacts with disialoganglioside and may be masked by cis interactions. Siglec-7 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-7 Protein, Human (HEK293, His) is 335 a.a., with molecular weight of 50-70 kDa.

Siglec-7 protein, identified as a putative adhesion molecule, serves as a mediator of sialic-acid dependent cellular binding. It exhibits a preference for binding to alpha-2,3- and alpha-2,6-linked sialic acid, along with interactions with disialogangliosides such as disialogalactosyl globoside, disialyl lactotetraosylceramide, and disialyl GalNAc lactotetraoslylceramide. Notably, the sialic acid recognition site of Siglec-7 may undergo masking due to cis interactions with sialic acids on the same cell surface. In immune responses, it functions as an inhibitory receptor, inducing tyrosine phosphorylation upon ligand binding and recruiting cytoplasmic phosphatases through their SH2 domains, thereby blocking signal transduction and inhibiting natural killer cell cytotoxicity. Siglec-7 may also play a role in hemopoiesis, inhibiting the differentiation of CD34+ cell precursors towards the myelomonocytic cell lineage and impeding the proliferation of leukemic myeloid cells in vitro. Additionally, it interacts with PTPN6/SHP-1 upon phosphorylation.

Human

HEK293

C-6*His

Q9Y286 (Q19-L353)

27036  [NCBI]

QKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLL

50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.