Siglec-8 Protein, Human (Biotinylated, HEK293, His-Avi)
Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Siglec-8 protein is a putative adhesion molecule that mediates sialic acid-dependent binding to erythrocytes, preferring α-2,3-linked sialic acid and interacting with α-2,6-linked sialic acid . Its sialic acid recognition site may be hidden through cis interactions with sialic acid on the surface of the same cell. Siglec-8 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-8 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
Siglec-8 protein, identified as a putative adhesion molecule, serves as a mediator for sialic-acid dependent binding to red blood cells. It exhibits a preference for binding to alpha-2,3-linked sialic acid and also interacts with alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-8 may be concealed due to cis interactions with sialic acids present on the same cell surface. Furthermore, it has the ability to simultaneously recognize epitopes featuring a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose, with a specific affinity for Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q9P0M4 (M17-A363)
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Molecular Construction
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N-term
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Siglec-8 (M17-A363)
Accession # Q9P0M4 -
8*His-Avi
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C-term
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Protein Length
Partial
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Conjugation
Biotin
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Synonyms
SIGLEC8; Sialoadhesin Family Member 2; Sialic Acid Binding Ig Like Lectin 8; MGC59785; SAF2; SAF-2; SIGLEC-8; Sialic Acid Binding Ig-Like Lectin 8; SIGLEC8L; Siglec-8; Sialic Acid-Binding Ig-Like Lectin 8; CDw329
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AA Sequence
MEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAA
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Predicted Molecular Mass
40.7 kDa
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Molecular Weight
Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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