Siglec-9 Protein, Human (HEK293, His-Avi)
Siglec-9 Protein, a putative adhesion molecule, mediates sialic-acid dependent cellular binding with a preference for alpha-2,3- or alpha-2,6-linked sialic acid. Its sialic acid recognition site may undergo masking through cis interactions with sialic acids on the same cell surface. Siglec-9 Protein, Human (HEK293, His-Avi) is the recombinant human-derived Siglec-9 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Siglec-9 Protein, a putative adhesion molecule, mediates sialic-acid dependent cellular binding with a preference for alpha-2,3- or alpha-2,6-linked sialic acid. Its sialic acid recognition site may undergo masking through cis interactions with sialic acids on the same cell surface. Siglec-9 Protein, Human (HEK293, His-Avi) is the recombinant human-derived Siglec-9 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
Siglec-9 protein, identified as a putative adhesion molecule, serves as a mediator in sialic-acid dependent cellular binding. It exhibits a preference for binding to alpha-2,3- or alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-9 may undergo masking due to cis interactions with sialic acids on the same cell surface.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q9Y336-1 (Q18-G348)
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Molecular Construction
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N-term
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Siglec-9 (Q18-G348)
Accession # Q9Y336-1 -
8*His-Avi
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C-term
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Protein Length
Extracellular Domain
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Synonyms
SIGLEC9; CDw329; Sialic Acid Binding Ig Like Lectin 9; Sialic Acid Binding Ig-Like Lectin 9; CD329; CD329 Antigen; Sialic Acid-Binding Ig-Like Lectin 9; OBBP-LIKE; Protein FOAP-9; FOAP-9; Siglec-9
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AA Sequence
QTSKLLTMQSSVTVQEGLCVHVPCSFSYPSHGWIYPGPVVHGYWFREGANTDQDAPVATNNPARAVWEETRDRFHLLGDPHTKNCTLSIRDARRSDAGRYFFRMEKGSIKWNYKHHRLSVNVTALTHRPNILIPGTLESGCPQNLTCSVPWACEQGTPPMISWIGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDAAEFTCRAQNPLGSQQVYLNVSLQSKATSGVTQG
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Predicted Molecular Mass
38.9 kDa
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Molecular Weight
Approximately 70-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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