SIRP gamma Protein, Human (HEK293, His-Avi)

SIRP γ protein is a possible immunoglobulin-like cell surface receptor that mediates cell-cell adhesion by binding to CD47. This interaction enhances antigen-specific T cell proliferation and provides costimulatory signals for T cell activation, highlighting the role of SIRP gamma in regulating immune responses. SIRP gamma Protein, Human (HEK293, His-Avi) is the recombinant human-derived SIRP gamma protein, expressed by HEK293 , with C-His, C-Avi labeled tag.

The SIRP gamma protein emerges as a probable immunoglobulin-like cell surface receptor, demonstrating its role in mediating cell-cell adhesion upon binding with CD47. This interaction is pivotal in enhancing antigen-specific T-cell proliferation and providing costimulatory signals for T-cell activation. As a result, SIRP gamma engages with CD47 on antigen-presenting cells, highlighting its involvement in modulating immune responses. The intricate interactions between SIRP gamma and CD47 underscore its significance as a cell surface receptor, playing a crucial role in cellular adhesion and the regulation of T-cell activation pathways.

Immobilized Human SIRP Gamma, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC₅₀ of 2.25 μg/ml determined by ELISA.

Human

HEK293

C-Avi;C-8*His

Q9P1W8-1 (E29-P360)

55423  [NCBI]

Extracellular Domain

EEELQMIQPEKLLLVTVGKTATLHCTVTSLLPVGPVLWFRGVGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRISSITPADVGTYYCVKFRKGSPENVEFKSGPGTEMALGAKPSAPVVLGPAARTTPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGDPLRGTANLSEAIRVPPTLEVTQQPMRVGNQVNVTCQVRKFYPQSLQLTWSENGNVCQRETASTLTENKDGTYNWTSWFLVNISDQRDDVVLTCQVKHDGQLAVSKRLALEVTVHQKDQSSDATP

Approximately 45-52 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.