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  5. SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His)

SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His)

Cat. No.: HY-P72466
Handling Instructions

SLAMF2/CD48 protein is a GPI-anchored glycoprotein that regulates immune cell function by interacting with CD2 and CD244. SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His) is the recombinant mouse-derived SLAMF2/CD48 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His) is 194 a.a., with molecular weight of 45-50 kDa.

For research use only. We do not sell to patients.

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Description

SLAMF2/CD48 protein is a GPI-anchored glycoprotein that regulates immune cell function by interacting with CD2 and CD244. SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His) is the recombinant mouse-derived SLAMF2/CD48 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His) is 194 a.a., with molecular weight of 45-50 kDa.

Background

The SLAMF2/CD48 Protein, a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein, plays a pivotal role in immune cell regulation and activation by interacting through its N-terminal immunoglobulin domain with cell surface receptors, such as 2B4/CD244 or CD2. In T-cell signaling transduction, SLAMF2 associates with CD2, facilitating the efficient recruitment of the Src family protein kinase LCK and LAT to the TCR/CD3 complex, thereby promoting LCK phosphorylation and subsequent activation. Furthermore, SLAMF2 induces the phosphorylation of the cytoplasmic immunoreceptor tyrosine switch motifs (ITSMs) of CD244, initiating a cascade of signaling events that culminate in the formation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells. Notably, SLAMF2 interacts directly with CD2, CD244, and LCK, highlighting its intricate involvement in immune cell function and signaling pathways.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q18PH8 (F23-R216)

Gene ID

12506  [NCBI]

Molecular Construction
N-term
CD48 (F23-R216)
Accession # Q18PH8
6*His
C-term
Synonyms
CD48; BLAST1; BCM1; SLAMF2; SLAM Family Member 2
AA Sequence

FQGHSIPDINATTGSNVTLKIHKDPLGPYKRITWLHTKNQKILEYNYNSTKTIFESEFKGRVYLEENDGALHISNVRKEDKGTYYMRVLRETENELKITLEVFDPVPKPSIEINKTEASTDSCHLRLSCEVKDQHVDYTWYESSGPFPKKSPGYVLDLIVTPQNKSTFYTCQVSNPVSSKNDTVYFTLPCDLAR

Molecular Weight

45-50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SLAMF2/CD48 Protein, Mouse (194a.a, HEK293, His)
Cat. No.:
HY-P72466
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