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SMPD1 Protein, Mouse (sf9, C-His)

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, C-His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SMPD1 Protein, Mouse (sf9, C-His)
  • SMPD1 Protein, Mouse (sf9, C-His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The SMPD1 protein converts sphingomyelin to ceramide through two enzymatic forms, one targeted to endolysosomes and the other released extracellularly.Lysosomal exocytosis becomes a significant source of the secretory form during stress responses.SMPD1 also exports cholesterol from intraendolysosomal membranes and exhibits phospholipase C activities.Its role in stress-induced apoptosis modulation is mediated through ceramide production.SMPD1 Protein, Mouse (sf9, C-His) is the recombinant mouse-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

The SMPD1 protein functions by converting sphingomyelin into ceramide through two enzymatic forms derived from alternative trafficking of a single precursor protein. One form is targeted to the endolysosomal compartment, while the other is released extracellularly. However, during stress responses, lysosomal exocytosis may serve as a significant source of the secretory form. Within lysosomes, it carries out the conversion of sphingomyelin to ceramide and plays a crucial role in exporting cholesterol from intraendolysosomal membranes. Additionally, SMPD1 exhibits phospholipase C activities towards 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Its involvement in stress-induced apoptosis modulation is mediated through ceramide production.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source Sf9 insect cells
  • Tag C-10*His
  • Accession

    Q04519 (L45-L626)

  • Gene ID
    20597
  • Molecular Construction
    • N-term
    • SMPD1 (M1-L626)
      Accession # Q04519
    • His
    • C-term
  • Protein Length

    Full Length of Sphingomyelin phosphodiesterase Chain

  • Synonyms

    SMPD1; Niemann-Pick Type A/B; Sphingomyelin Phosphodiesterase 1; Sphingomyelin Phosphodiesterase 1 Isoform 7; Acid Sphingomyelinase; SMPD1 Protein; ASM; ASMASE; Sphingomyelin Phosphodiesterase; ASMase; Sphingomyelin Phosphodiesterase 1, Acid Lysosomal; NP

  • AA Sequence

    LFDSTVLWVPARAYPLPSEGHSVKFSAIAPPLQSAFGWQNLTCPACKVLFTALNHGLKKEPNVARVGSVAIKICKMLNIAPLDVCQSAVHLFEDDVVEVWTRSVLSPSEACGLLLGSSCGHWDIFSTWNISLPSVPKPPPKPPSPPAPGAPVSRVLFLTDLHWDHEYLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLPLRTLESLLKGLGPAGPFEMVYWTGDIPAHDVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFIKGNQSSQWLYEAMAKAWEPWLPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHIIGHIPPGHCLKSWSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDEETLSRPLAVAFLAPSATTFINLNPGYRVYQIDGNYPGSSHVVLDHETYILNLTQANAAGGTPSWKRLYRARETYGLPDAMPASWHNLVYRMRDDEQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPNGSLPDANRLWSRPLL

  • Predicted Molecular Mass

    65 kDa

  • Molecular Weight

    Approximately 68-71 kDa, based on SDS-PAGE under reducing conditions,due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • SMPD1 Protein, Mouse (sf9, C-His) Product Image
    1/16
  • Experimental Validation Results for SMPD1 Protein, Mouse (sf9, C-His)
  • SMPD1 Protein, Mouse (sf9, C-His) Product Image