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SMPDL3A Protein, Human (HEK293, His)

Cat. No.: HY-P71325
Handling Instructions

SMPDL3A exhibits in vitro nucleotide phosphodiesterase activity, specifically toward ATP and p-nitrophenyl-TMP, less activity toward nucleoside diphosphates, and no activity toward nucleoside monophosphates. Notably, it showed in vitro activity with CDP-choline and CDP-ethanolamine, producing specific products. SMPDL3A Protein, Human (HEK293, His) is the recombinant human-derived SMPDL3A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SMPDL3A Protein, Human (HEK293, His) is 431 a.a., with molecular weight of ~59.0 kDa.

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Description

SMPDL3A exhibits in vitro nucleotide phosphodiesterase activity, specifically toward ATP and p-nitrophenyl-TMP, less activity toward nucleoside diphosphates, and no activity toward nucleoside monophosphates. Notably, it showed in vitro activity with CDP-choline and CDP-ethanolamine, producing specific products. SMPDL3A Protein, Human (HEK293, His) is the recombinant human-derived SMPDL3A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SMPDL3A Protein, Human (HEK293, His) is 431 a.a., with molecular weight of ~59.0 kDa.

Background

SMPDL3A exhibits in vitro nucleotide phosphodiesterase activity, particularly with nucleoside triphosphates such as ATP, as well as p-nitrophenyl-TMP. While it displays lower activity with nucleoside diphosphates, no activity is observed with nucleoside monophosphates. Notably, SMPDL3A demonstrates in vitro activity with CDP-choline, producing CMP and phosphocholine, as well as with CDP-ethanolamine. However, it does not possess sphingomyelin phosphodiesterase activity. These enzymatic characteristics highlight the substrate specificity and potential physiological relevance of SMPDL3A in nucleotide phosphodiester hydrolysis.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q92484 (L23-Y453)

Gene ID
Molecular Construction
N-term
SMPDL3A (L23-Y453)
Accession # Q92484
6*His
C-term
Synonyms
Acid sphingomyelinase-like phosphodiesterase 3a; ASM-like phosphodiesterase 3a; SMPDL3A; ASML3A
AA Sequence

LPVAPAGGRNPPPAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY

Molecular Weight

Approximately 59.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

SMPDL3A Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SMPDL3A Protein, Human (HEK293, His)
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HY-P71325
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