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  3. SPARCL1 Protein, Mouse (283a.a, HEK293, His)

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (283a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with N-His labeled tag.

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Description

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (283a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with N-His labeled tag.

Background

SPARCL1 protein is a multifunctional molecule with calcium ion, collagen and extracellular matrix binding activities. It is mainly localized at glutamatergic synapses and participates in the synaptic membrane adhesion process. SPARCL1 is widely expressed in multiple systems, including cardiovascular, central nervous, male reproductive, respiratory and sensory organ systems, and is particularly highly expressed in adult bladder and cortex, suggesting that it plays a key role in these tissues. The protein is highly conserved in evolution, and the existence of its human homolog SPARC like 1 further confirms its functional importance.

Species

Mouse

Source

HEK293

Tag

N-6*His

Accession

NP_034227.3 (Y368-F650)

Gene ID
Molecular Construction
N-term
His
SPARCL1 (Y368-F650)
Accession # NP_034227.3
C-term
Protein Length

Partial

Synonyms
SPARC-like protein 1; Extracellular matrix protein 2; Matrix glycoprotein Sc1; ECM2, SC1
AA Sequence

YLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Predicted Molecular Mass
32.4 kDa
Molecular Weight

Approximately 38-48 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity
  • ≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

SPARCL1 Protein, Mouse (283a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SPARCL1 Protein, Mouse (283a.a, HEK293, His)

 

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Product Name:
SPARCL1 Protein, Mouse (283a.a, HEK293, His)
Cat. No.:
HY-P76652
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