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  4. SPINK1 Protein, Human (HEK293, His)

SPINK1 Protein, Human (HEK293, His)

Cat. No.: HY-P71053
COA Handling Instructions

The SPINK1 protein is a serine protease inhibitor that significantly inhibits trypsin, especially in the pancreas, preventing premature activation of the zymogen. This critical role maintains the integrity of pancreatic cellular processes. SPINK1 Protein, Human (HEK293, His) is the recombinant human-derived SPINK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SPINK1 Protein, Human (HEK293, His) is 56 a.a., with molecular weight of 12-16 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The SPINK1 protein is a serine protease inhibitor that significantly inhibits trypsin, especially in the pancreas, preventing premature activation of the zymogen. This critical role maintains the integrity of pancreatic cellular processes. SPINK1 Protein, Human (HEK293, His) is the recombinant human-derived SPINK1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SPINK1 Protein, Human (HEK293, His) is 56 a.a., with molecular weight of 12-16 kDa.

Background

SPINK1, a serine protease inhibitor, demonstrates notable anti-trypsin activity, particularly in the pancreas where it serves a critical role in safeguarding against premature activation of zymogens catalyzed by trypsin. This protective function helps maintain the integrity of cellular processes within the pancreas. Additionally, SPINK1 exhibits involvement in the male reproductive tract, where it binds to sperm heads, exerting its influence on sperm capacitance. Notably, SPINK1 achieves this modulation by inhibiting calcium uptake and nitrogen oxide (NO) production, showcasing its diverse regulatory functions in distinct physiological contexts.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P00995 (D24-C79)

Gene ID
Molecular Construction
N-term
SPINK1 (D24-C79)
Accession # P00995
6*His
C-term
Synonyms
Pancreatic Secretory Trypsin Inhibitor; Serine Protease Inhibitor Kazal-Type 1; Tumor-Associated Trypsin Inhibitor; TATI; SPINK1; PSTI
AA Sequence

DSLGREAKCYNELNGCTKIYDPVCGTDGNTYPNECVLCFENRKRQTSILIQKSGPC

Molecular Weight

12-16 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, pH 9.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

SPINK1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SPINK1 Protein, Human (HEK293, His)
Cat. No.:
HY-P71053
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