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SRFBP1 Protein, Human (sf9, His-GST)

The SRFBP1 protein is a potential regulator of multiple cellular processes and is involved in aging-associated transcriptional activation of cardiac genes and the biosynthesis/processing of SLC2A4 in adipocytes. Its multifaceted effects extend into metabolic pathways, involving interactions with SRF and formation of complexes with SRF cofactors ARID2, MYOCD, and NKX2-5, highlighting its role in transcriptional regulation. SRFBP1 Protein, Human (sf9, His-GST) is the recombinant human-derived SRFBP1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
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Biological Activity

Description

The SRFBP1 protein is a potential regulator of multiple cellular processes and is involved in aging-associated transcriptional activation of cardiac genes and the biosynthesis/processing of SLC2A4 in adipocytes. Its multifaceted effects extend into metabolic pathways, involving interactions with SRF and formation of complexes with SRF cofactors ARID2, MYOCD, and NKX2-5, highlighting its role in transcriptional regulation. SRFBP1 Protein, Human (sf9, His-GST) is the recombinant human-derived SRFBP1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

SRFBP1 Protein emerges as a potential regulator in diverse cellular processes, with implications in the aging-associated transcriptional activation of cardiac genes. Additionally, it may play a role in the biosynthesis and/or processing of SLC2A4 in adipose cells, suggesting a multifaceted involvement in metabolic pathways. Interactions with the transcription factor SRF, as well as the formation of complexes with SRF cofactors ARID2, MYOCD, and NKX2-5, highlight SRFBP1's role in transcriptional regulation. Moreover, its interaction with the N-terminus of SLC2A4 suggests a potential role in the modulation of glucose transport processes. Unraveling the specific mechanisms governing SRFBP1's functions could provide valuable insights into its role in cardiac aging, metabolic regulation, and transcriptional control, paving the way for a more comprehensive understanding of its cellular impact.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His;N-GST
  • Accession

    Q8NEF9 (M1-D429)

  • Gene ID
    153443  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • SRFBP1 (M1-D429)
      Accession # Q8NEF9
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    SRFBP1; SRF-Dependent Transcription Regulation-Associated Protein; Serum Response Factor Binding Protein 1; Serum Response Factor-Binding Protein 1; STRAP; P49/STRAP; BUD22; FLJ25286; Rlb1; BUD22 Homolog (S. Cerevisiae); P49; BUD22 Homolog

  • AA Sequence

    MAQPGTLNLNNEVVKMRKEVKRIRVLVIRKLVRSVGRLKSKKGTEDALLKNQRRAQRLLEEIHAMKELKPDIVTKSALGDDINFEKIFKKPDSTATERAIARLAVHPLLKKKIDVLKAAVQAFKEARQNVAEVESSKNASEDNHSENTLYSNDNGSNLQREATVISEQKVKETKILAKKPIHNSKEKIAKMEHGPKAVTIANSPSKPSEKDSVVSLESQKTPADPKLKTLSQTKKNKGSDSSLSGNSDGGEEFCEEEKEYFDDSTEERFYKQSSMSEDSDSGDDFFIGKVRRTRKKESSCHSSVKEQKPLEKVFLKEDTGETHGDTRNDKIKPSTETRKLESVFFHSLSGSKSSRRNFKEQAPKTRSLDFPQNEPQIKNQFNKKLSGRLENTKQQLQLPLHPSWEASRRRKEQQSNIAVFQGKKITFDD

  • Predicted Molecular Mass

    76.5 kDa

  • Molecular Weight

    Approximately 69 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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