SRPK2 Protein, Human (Active, sf9, GST)
SRPK2 is a serine/arginine-rich protein-specific kinase that phosphorylates serine residues within the arginine/serine-rich domain (RS domain) and plays a critical role in regulating the splicing process effect. SRPK2 promotes neuronal apoptosis by upregulating cyclin-D1 (CCND1) expression and inhibiting the inhibitory effect of p53/TP53. SRPK2 Protein, Human (sf9, GST) is the recombinant human-derived SRPK2 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SRPK2 is a serine/arginine-rich protein-specific kinase that phosphorylates serine residues within the arginine/serine-rich domain (RS domain) and plays a critical role in regulating the splicing process effect. SRPK2 promotes neuronal apoptosis by upregulating cyclin-D1 (CCND1) expression and inhibiting the inhibitory effect of p53/TP53. SRPK2 Protein, Human (sf9, GST) is the recombinant human-derived SRPK2 protein, expressed by sf9 insect cells , with N-GST labeled tag.
Background
SRPK2, a serine/arginine-rich protein-specific kinase, exhibits a specific penchant for phosphorylating serine residues within arginine/serine-rich domains, known as RS domains. Functionally, SRPK2 is integral to the phosphorylation of SR splicing factors, intricately regulating splicing processes. Notably, SRPK2 contributes to neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression, achieved through the phosphorylation of SRSF2. This phosphorylation event suppresses p53/TP53 phosphorylation, thus alleviating p53/TP53's repressive impact on cyclin-D1 (CCND1) expression. Additionally, SRPK2 plays a crucial role in spliceosomal B complex formation by phosphorylating DDX23/PRP28, and its phosphorylation of ACIN1 leads to the redistribution of ACIN1 from nuclear speckles to the nucleoplasm, resulting in up-regulation of cyclin A1. Furthermore, SRPK2 participates in modulating hepatitis B virus (HBV) replication, impacting HBV core protein phosphorylation and pregenomic RNA (pgRNA) packaging efficiency, revealing its multifaceted regulatory roles in cellular and viral processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P78362 (S2-N688)
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Gene ID6733
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Molecular Construction
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N-term
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GST
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SRPK2 (S2-N688)
Accession # P78362 -
C-term
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Protein Length
Full Length
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Synonyms
SRPK2; SR-Protein-Specific Kinase 2; SRSF Protein Kinase 2; SR Protein Kinase 2; SFRS Protein Kinase 2; Non-Specific Serine/Threonine Protein Kinase; Serine/Arginine-Rich Protein-Specific Kinase 2; Serine/Threonine-Protein Kinase SRPK2; SFRSK2; Uncharacte
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AA Sequence
SVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLN
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Molecular Weight
Approximately 135 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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