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STAT1 Protein, Human

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

STAT1 is a signal transducer and activator of transcription that responds to interferons, KITLG/SCF, and growth factors. After type I IFN binds, STAT1 and STAT2 form ISGF3, which translocates to the nucleus and activates ISG transcription. STAT1 Protein, Human is the recombinant human-derived STAT1 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for STAT1 Protein, Human
  • Experimental Validation Results for STAT1 Protein, Human
  • STAT1 Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

STAT1 is a signal transducer and activator of transcription that responds to interferons, KITLG/SCF, and growth factors. After type I IFN binds, STAT1 and STAT2 form ISGF3, which translocates to the nucleus and activates ISG transcription. STAT1 Protein, Human is the recombinant human-derived STAT1 protein, expressed by E. coli , with tag free.

Background

STAT1 is a signal transducer and transcription activator that plays a crucial role in mediating cellular responses to interferons (IFNs), cytokine KITLG/SCF, and various growth factors. Upon binding of type I IFN (IFN-alpha and IFN-beta) to cell surface receptors, STAT1 undergoes tyrosine phosphorylation and forms a dimer with STAT2. This complex, known as ISGF3 transcription factor, translocates to the nucleus and activates the transcription of IFN-stimulated genes (ISG), establishing an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is phosphorylated at both tyrosine and serine residues, forming a homodimer (GAF) that enters the nucleus and binds to the IFN-gamma activated sequence (GAS), driving the expression of target genes and inducing a cellular antiviral state. Additionally, STAT1 becomes activated in response to KITLG/SCF and KIT signaling and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3, and FGFR4. Furthermore, in the small intestine, STAT1 associates with Gasdermin-D and promotes the transcription of CIITA, inducing the formation of type 1 regulatory T (Tr1) cells. STAT1 interacts with various proteins, including PIAS1, IFNAR1, IFNAR2, NMI, CREBBP/CBP, PTK2/FAK1, SRC, ERBB4, PARP9, DTX3L, EP300/p300, and IFNGR1, contributing to its diverse cellular functions.

Verified Bioactivity

Measured in a cytotoxicity assay using HepG2 cells. The ED50 for this effect is 3.912 μg/mL, corresponding to a specific activity is 255.624 units/mg.

Results
  • Experimental Validation Results for STAT1 Protein, Human
    Measured in a cytotoxicity assay using HepG2 cells. The ED50 for this effect is 3.912 μg/mL, corresponding to a specific activity is 255.624 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P42224-2 (M1-V712)

  • Gene ID
    6772  [NCBI]
  • Molecular Construction
    • N-term
    • STAT1 (M1-V712)
      Accession # P42224-2
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    Signal Transducer and Activator of Transcription 1-Alpha/Beta; Transcription Factor ISGF-3 Components p91/p84; STAT1

  • AA Sequence

    MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEV

  • Predicted Molecular Mass

    83.3 kDa

  • Molecular Weight

    Approximately 85 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for STAT1 Protein, Human
      ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 0.02% Tween 80, 5% sucrose, 4% mannitol, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 85%, as determined by reducing SDS-PAGE.

  • Measured in a cytotoxicity assay using HepG2 cells. The ED50 for this effect is 3.912 μg/mL, corresponding to a specific activity is 255.624 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 85%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cytotoxicity assay using HepG2 cells. The ED50 for this effect is 3.912 μg/mL, corresponding to a specific activity is 255.624 units/mg.

  • STAT1 Protein, Human Product Image
    1/16
  • Experimental Validation Results for STAT1 Protein, Human
  • Experimental Validation Results for STAT1 Protein, Human
  • STAT1 Protein, Human Product Image