STAT4 Protein, Human (sf9, His)
Based on 1 Customer Validation
The STAT4 protein is mainly expressed in hematopoietic cells and is critical for cell growth, differentiation and immune responses. It plays a key role in T helper 1 cell differentiation, interferon gamma production, and various neutrophil activities. STAT4 Protein, Human (sf9, His) is the recombinant human-derived STAT4 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The STAT4 protein is mainly expressed in hematopoietic cells and is critical for cell growth, differentiation and immune responses. It plays a key role in T helper 1 cell differentiation, interferon gamma production, and various neutrophil activities. STAT4 Protein, Human (sf9, His) is the recombinant human-derived STAT4 protein, expressed by Sf9 insect cells , with N-His labeled tag.
STAT4, a transcriptional regulator predominantly expressed in hematopoietic cells, plays a crucial role in cellular growth, differentiation, and immune response. It is particularly involved in the differentiation of T-helper 1 cells and the production of interferon-gamma, showcasing its significance in orchestrating immune functions. STAT4's engagement in multiple neutrophil activities, including chemotaxis and neutrophil extracellular trap production, further underscores its versatile role. Upon IL12 binding to IL12RB2, STAT4 undergoes tyrosine phosphorylation, leading to homodimerization and nuclear translocation. While IL12 serves as the primary activating signal, STAT4 can also be activated in response to IFN-gamma, IL23, IL2, and IL35. In the context of IFN-alpha/beta signaling, STAT4 acts as a transcriptional repressor, suppressing IL5 and IL13 mRNA expression during T-cell receptor activation. This multifaceted regulatory protein forms homodimers or heterodimers with related family members and interacts with various partners, including ARL2BP, IL12RB2, STAT1, and JUN, contributing to its intricate role in immune modulation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
Q14765 (M1-E748)
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Molecular Construction
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N-term
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His
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STAT4 (M1-E748)
Accession # Q14765 -
C-term
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Protein Length
Full Length
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Synonyms
STAT4; Signal Transducer And Activator Of Transcription; Signal Transducer And Activator Of Transcription 4; SLEB11; Signal Transducer And Activator Of Transcription 4 Variant 3; DPMC
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AA Sequence
MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE
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Predicted Molecular Mass
87.8 kDa
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Molecular Weight
Approximately 70-88 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% glycerol, 0.5 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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