STAT4 Protein, Human (sf9, His)

The STAT4 protein is mainly expressed in hematopoietic cells and is critical for cell growth, differentiation and immune responses. It plays a key role in T helper 1 cell differentiation, interferon gamma production, and various neutrophil activities. STAT4 Protein, Human (sf9, His) is the recombinant human-derived STAT4 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of STAT4 Protein, Human (sf9, His) is 748 a.a., with molecular weight of ~87 kDa.

STAT4, a transcriptional regulator predominantly expressed in hematopoietic cells, plays a crucial role in cellular growth, differentiation, and immune response. It is particularly involved in the differentiation of T-helper 1 cells and the production of interferon-gamma, showcasing its significance in orchestrating immune functions. STAT4's engagement in multiple neutrophil activities, including chemotaxis and neutrophil extracellular trap production, further underscores its versatile role. Upon IL12 binding to IL12RB2, STAT4 undergoes tyrosine phosphorylation, leading to homodimerization and nuclear translocation. While IL12 serves as the primary activating signal, STAT4 can also be activated in response to IFN-gamma, IL23, IL2, and IL35. In the context of IFN-alpha/beta signaling, STAT4 acts as a transcriptional repressor, suppressing IL5 and IL13 mRNA expression during T-cell receptor activation. This multifaceted regulatory protein forms homodimers or heterodimers with related family members and interacts with various partners, including ARL2BP, IL12RB2, STAT1, and JUN, contributing to its intricate role in immune modulation.

Human

Sf9 insect cells

N-His

Q14765 (M1-E748)

6775  [NCBI]

Approximately 87 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% glycerol, 0.5 mM DTT.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.