SUV420H2 Protein, Human (His)
Based on 1 Customer Validation
SUV420H2 protein is a histone methyltransferase that methylates H4K20 to generate H4K20me3 and H4K20me2, which are critical for transcriptional regulation and genome integrity. SUV420H2 functions in pericentric heterochromatin, establishes constitutive heterochromatin, and interacts with RB1 family proteins. SUV420H2 Protein, Human (His) is the recombinant human-derived SUV420H2 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SUV420H2 protein is a histone methyltransferase that methylates H4K20 to generate H4K20me3 and H4K20me2, which are critical for transcriptional regulation and genome integrity. SUV420H2 functions in pericentric heterochromatin, establishes constitutive heterochromatin, and interacts with RB1 family proteins. SUV420H2 Protein, Human (His) is the recombinant human-derived SUV420H2 protein, expressed by E. coli , with N-His labeled tag.
Background
SUV420H2 Protein, a histone methyltransferase, exhibits specificity in methylating monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4, producing trimethylated 'Lys-20' (H4K20me3) and dimethylated 'Lys-20' (H4K20me2), thus contributing to the regulation of transcription and maintenance of genome integrity. In vitro, it also demonstrates the ability to methylate unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation serves as a specific epigenetic tag associated with transcriptional repression. Operating predominantly in pericentric heterochromatin regions, SUV420H2 plays a central role in establishing constitutive heterochromatin in these genomic areas. Targeted to histone H3 through interaction with RB1 family proteins (RB1, RBL1, and RBL2), the enzyme facilitates TP53BP1 foci formation upon DNA damage, promoting proficient non-homologous end-joining (NHEJ)-directed DNA repair through the di- and trimethylation of 'Lys-20' of histone H4. Additionally, it may contribute to class switch recombination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q86Y97-1 (G2-L280)
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Molecular Construction
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N-term
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His
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SUV420H2 (G2-L280)
Accession # Q86Y97-1 -
C-term
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Protein Length
Partial
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Synonyms
KMT5C; Lysine-Specific Methyltransferase 5C; Prev. SUV420H2; Su(Var)4-20 Homolog 2; [Histone H4]-N-Methyl-L-Lysine20 N-Methyltransferase KMT5B; Suv4-20h2; [Histone H4]-Lysine20 N-Methyltransferase KMT5B; MGC2705; Lysine (K)-Specific Methyltransferase 5C;
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AA Sequence
GPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTLGGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGL
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Predicted Molecular Mass
31.7 kDa
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Molecular Weight
Approximately 32 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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