SUV420H2 Protein, Human (His)

SUV420H2 protein is a histone methyltransferase that methylates H4K20 to generate H4K20me3 and H4K20me2, which are critical for transcriptional regulation and genome integrity. SUV420H2 functions in pericentric heterochromatin, establishes constitutive heterochromatin, and interacts with RB1 family proteins. SUV420H2 Protein, Human (His) is the recombinant human-derived SUV420H2 protein, expressed by E. coli , with N-His labeled tag. The total length of SUV420H2 Protein, Human (His) is 279 a.a., with molecular weight of ~60 kDa.

SUV420H2 Protein, a histone methyltransferase, exhibits specificity in methylating monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4, producing trimethylated 'Lys-20' (H4K20me3) and dimethylated 'Lys-20' (H4K20me2), thus contributing to the regulation of transcription and maintenance of genome integrity. In vitro, it also demonstrates the ability to methylate unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation serves as a specific epigenetic tag associated with transcriptional repression. Operating predominantly in pericentric heterochromatin regions, SUV420H2 plays a central role in establishing constitutive heterochromatin in these genomic areas. Targeted to histone H3 through interaction with RB1 family proteins (RB1, RBL1, and RBL2), the enzyme facilitates TP53BP1 foci formation upon DNA damage, promoting proficient non-homologous end-joining (NHEJ)-directed DNA repair through the di- and trimethylation of 'Lys-20' of histone H4. Additionally, it may contribute to class switch recombination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).

Human

E. coli

N-His

Q86Y97 (G2-L280)

84787  [NCBI]

GPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTLGGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGL

Approximately 60 kDa

Greater than 80% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.