Syntaxin-7 Protein, Rat (HEK293, His)
Based on 1 Customer Validation
Syntaxin-7 protein is a central player in protein trafficking and membrane fusion events. It guides proteins from the plasma membrane to early endosomes, facilitates homotypic fusion of endocytic organelles, and mediates transport from early endosomes to late endosomes and lysosomes. Syntaxin-7 Protein, Rat (HEK293, His) is the recombinant rat-derived Syntaxin-7 protein, expressed by HEK293 , with N-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Syntaxin-7 protein is a central player in protein trafficking and membrane fusion events. It guides proteins from the plasma membrane to early endosomes, facilitates homotypic fusion of endocytic organelles, and mediates transport from early endosomes to late endosomes and lysosomes. Syntaxin-7 Protein, Rat (HEK293, His) is the recombinant rat-derived Syntaxin-7 protein, expressed by HEK293 , with N-His labeled tag.
Background
Syntaxin-7 Protein emerges as a key player in cellular processes related to protein trafficking and membrane fusion events. It is implicated in the trafficking of proteins from the plasma membrane to the early endosome (EE) and contributes to the homotypic fusion of endocytic organelles. Additionally, Syntaxin-7 plays a pivotal role in mediating endocytic trafficking from early endosomes to late endosomes and lysosomes. Interactions with VPS11, VPS16, VPS18, and VPS33A underscore its involvement in endosomal dynamics. Furthermore, Syntaxin-7 forms a SNARE complex with VTI1B, STX8, and VAMP8, crucial for the homotypic fusion of late endosomes. It is also a component of the SNARE complex with STX7, STX8, VAMP7, and VTI1B, essential for heterotypic fusion of late endosomes with lysosomes. The interaction with TPC1 further suggests its participation in intricate cellular processes. Investigating the detailed mechanisms underlying Syntaxin-7's interactions and functions could provide valuable insights into its role in membrane trafficking and fusion events within the endocytic pathway.
Verified Bioactivity
Immobilized Rat Syntaxin-7 at 2 μg/mL (100 μL/well) can bind Anti- Syntaxin-7 Antibody, The ED50 for this effect is 0.01394 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Rat
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Source HEK293
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Tag N-6*His
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Accession
O70257/NP_068641.2 (S2-L238)
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Molecular Construction
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N-term
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His
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Syntaxin-7 (S2-L238)
Accession # O70257/NP_068641.2 -
C-term
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Protein Length
Cytoplasmic Domain
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Synonyms
STX7; Syntaxin-7; Syntaxin 7; CDNA FLJ50258, Weakly Similar To Syntaxin-7
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AA Sequence
SYTPGIGGDPAQLAQRISSNIQKITQCSAEIQRTLNQLGTPQDTPELRQQLQQEQQYTNQLAKETDKYIKEFGFLPTTPSEQRQRKIQKDRLVAEFTTALTNFQKVQRQAAEREKEFVARVRASSRVSGGFPEDSSKEKNFVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVESAEVHVQQANQQLSRAANYQRKSRKTL
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Predicted Molecular Mass
27.2 kDa
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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