TAF1 Protein, Human (BD1, His)
TAF1 Protein, Human (BD1, His) is the recombinant human-derived TAF1, expressed by E. coli , with N-6*His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TAF1 Protein, Human (BD1, His) is the recombinant human-derived TAF1, expressed by E. coli , with N-6*His labeled tag. ,
TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex, involved in nucleating complex assembly. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, promoting assembly of the pre-initiation complex (PIC). TAF1 forms a promoter DNA binding subcomplex of TFIID together with TAF7 and TAF2. It contains novel N- and C-terminal Ser/Thr kinase domains that can autophosphorylate or transphosphorylate other transcription factors, such as phosphorylating TP53 at Thr-55 leading to MDM2-mediated degradation of TP53, and phosphorylating GTF2A1 and GTF2F1 at Ser residues. TAF1 also possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle. Additionally, TAF1 exhibits histone acetyltransferase activity towards histones H3 and H4.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P21675-2 (T1400-P1518)
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Gene ID6872
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Molecular Construction
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N-term
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6*His
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TAF1 (T1400-P1518)
Accession # P21675-2 -
C-term
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Protein Length
Partial
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Synonyms
TAF1; TAF(II)250; Prev. TAF2A; P250; Prev. BA2R; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, A, 250kD; Prev. CCG1; Transcription Initiation Factor TFIID 250 KDa Subunit; Prev. CCGS; Transcription Initiation Factor TFIID Subunit; P
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AA Sequence
TDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINP
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Predicted Molecular Mass
14.9 kDa
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Molecular Weight
Approximately 14 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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