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TAG-72 Protein, Canine (His)

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TAG-72 Protein, Canine (His) is the recombinant canine-derived TAG-72 protein, expressed by E. coli, with C-His tag.

For research use only. We do not sell to patients.
  • Species: Canine
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • TAG-72 Protein, Canine (His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

TAG-72 Protein, Canine (His) is the recombinant canine-derived TAG-72 protein, expressed by E. coli, with C-His tag.

Background

TAG-72 is the catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex, which methylates the N7 position of the guanosine added to the 5'-cap structure of mRNAs. It binds RNA containing a 5'-terminal GpppC.

Technical Parameters

  • Species Canine
  • Source E. coli
  • Tag C-8*His
  • Accession

    XP_005615459 (M1-Q478)

  • Gene ID
    476200
  • Protein Length

    Full Length

  • Synonyms

    RNMT; HCMT1; RNA Guanine-7 Methyltransferase; Hcm1p; RG7MT1; HMet; N7-MTase; MRNA (Guanine-N(7)-)-Methyltransferase; MRNA Cap Guanine-N(7) Methyltransferase; MRNA (Guanine-7-)Methyltransferase; MRNA Cap Guanine-N7 Methyltransferase; KIAA0398; MRNA (Guanin

  • AA Sequence

    MENSAKAEECEKLSLEEAKASKDLETKSSFSINENTTTPGTGLSEKAPVWEQADAPKKRKMEFEDDLIKESSSCGEDTPTKKRKLDAEIVPEEKGSGDDEGISRKRRMETDDFPKDESSTGDGTQKKRKIELEDVPEKQKNLEEGHSSAVAAHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRCRDNEYIFNAEFVTADCSKELLFNKFRDPETCFDICSCQFVCHYSFESYEQADMMLRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGDYPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNNENKMLLKRMQALEPYPANENSKLASEKVDDYEHAAEYMKNSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ

  • Predicted Molecular Mass

    56.2 kDa

  • Molecular Weight

    Approximately 56 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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