TCPTP Protein, Mouse (sf9, His)

TCPTP is a non-receptor tyrosine-specific phosphatase that regulates multiple signaling pathways by dephosphorylating proteins such as INSR, EGFR, CSF1R, PDGFR, JAK, Src kinase and STAT.TCPTP acts in the nucleus and cytoplasm to regulate hematopoiesis, inflammation, cell proliferation, differentiation, and glucose homeostasis.TCPTP Protein, Mouse (sf9, His) is the recombinant mouse-derived TCPTP protein, expressed by Sf9 insect cells , with N-His labeled tag.

TCPTP, a non-receptor type tyrosine-specific phosphatase, exerts its regulatory influence by dephosphorylating a spectrum of proteins involved in various signaling pathways. This includes receptor protein tyrosine kinases like INSR, EGFR, CSF1R, and PDGFR, as well as non-receptor protein tyrosine kinases such as JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3, and STAT6. Operating both in the nucleus and cytoplasm, TCPTP negatively modulates crucial biological processes, spanning hematopoiesis, inflammatory response, cell proliferation, and differentiation, as well as glucose homeostasis. It plays a pivotal role in immune system development, impacting T-cell receptor signaling, macrophage differentiation, and cytokine-mediated pathways. Additionally, TCPTP participates in the negative regulation of growth factor signaling, exemplified by its role in anchorage-dependent control of EGF-stimulated cell growth. Activated by integrin ITGA1/ITGB1, TCPTP dephosphorylates EGFR, PDGFRB, and SRC, exerting regulatory effects on their respective signaling pathways. Notably, TCPTP extends its influence to glucose homeostasis by negatively regulating insulin receptor signaling and controlling gluconeogenesis. Moreover, it may exhibit DNA-binding capabilities.

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate and the specific activity is > 15 nmoles/min/μg.

Mouse

Sf9 insect cells

N-His

Q06180 (S2-N314)

19255  [NCBI]

Partial

SATIEREFEELDAQCRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQSTENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRTVEKESVKCAQYWPTDDREMVFKETGFSVKLLSEDVKSYYTVHLLQLENINTGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGCLTPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGEDVNVKQLLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKYTKGDSNIQKRWKELSKEDLSPICDHSQNRVMVEKYN

Approximately 42 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 20% Glycerol, 0.1 mM TCEP, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.