1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily Neurotrophic Factors
  4. Transforming Growth Factor-β TGF- β
  5. TGF-β1
  6. TGF beta 1/TGFB1 Protein, Human (C33S, 361a.a, HEK293, His)

TGF beta 1/TGFB1 Protein, Human (C33S, 361a.a, HEK293, His)

Cat. No.: HY-P70236
COA Handling Instructions

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. TGF beta 1/TGFB1 Protein, Human (361a.a, HEK293, His) is a recombinant Biotinylated protein (L30-S390) produced by HEK293 cells with His tag.

For research use only. We do not sell to patients.

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Description

Latent TGF beta 1/TGFB1 Protein is a large extracellular matrix protein and an associated ligand of fibrillinmicrofibrils. TGF beta 1/TGFB1 Protein, Human (361a.a, HEK293, His) is a recombinant Biotinylated protein (L30-S390) produced by HEK293 cells with His tag[1][2].

Background

Latent TGF beta 1/TGFB1 Protein promotes fibrillin-1 and -2 assembly downstream of fibronectin in mouse embryonic fibroblasts[3].
Latent TGF beta 1/TGFB1 Protein promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fbroblasts transformation.[4].
Latent TGF beta 1/TGFB1 Protein plays a potential bridge between depressive disorder and glioblastoma[5].

In Vitro

LTBP-1 fragments (30 µg/mL) inhibit the incorporation of native LTBP-1 into fibroblast matrix[2].

Species

Human

Source

HEK293

Tag

N-6*His

Accession

P01137 (L30-S390,C33S)

Gene ID
Molecular Construction
N-term
6*His
TGFB1 (L30-S390,C33S)
Accession # P01137
C-term
Synonyms
rHuLatent TGF Beta-1-C33S, His; Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
AA Sequence

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Molecular Weight

38-55&13 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TGF beta 1/TGFB1 Protein, Human (C33S, 361a.a, HEK293, His)
Cat. No.:
HY-P70236
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