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TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

TGFBR3/TGF-beta RIII Protein is pivotal, binding to TGF-beta, indicating a potential role in capturing and presenting TGF-beta to signaling receptors.In gonadotrope cells, it acts as an inhibin A coreceptor, regulating FSH levels and impacting female fertility.Its interaction with DYNLT4 emphasizes diverse functional associations in cellular processes, suggesting a multifaceted role for TGFBR3 in cellular regulation and fertility control. TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His) is the recombinant mouse-derived TGFBR3/TGF-beta RIII protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His)
  • Experimental Validation Results for TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His)
  • TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

TGFBR3/TGF-beta RIII Protein is pivotal, binding to TGF-beta, indicating a potential role in capturing and presenting TGF-beta to signaling receptors.In gonadotrope cells, it acts as an inhibin A coreceptor, regulating FSH levels and impacting female fertility.Its interaction with DYNLT4 emphasizes diverse functional associations in cellular processes, suggesting a multifaceted role for TGFBR3 in cellular regulation and fertility control. TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His) is the recombinant mouse-derived TGFBR3/TGF-beta RIII protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The TGFBR3/TGF-beta RIII protein plays a pivotal role as it binds to TGF-beta, suggesting potential involvement in capturing and retaining TGF-beta for presentation to the signaling receptors, as inferred by similarity to related proteins. Particularly in gonadotrope cells, TGFBR3 acts as an inhibin A coreceptor, exerting regulatory control over follicle-stimulating hormone (FSH) levels and influencing female fertility, as indicated in relevant studies. Furthermore, TGFBR3 interacts with DYNLT4, underscoring its diverse functional associations in cellular processes.

Verified Bioactivity

Measured by its ability to inhibit TGF-beta 2 activity on HT-2 mouse T cells. The ED50 for this effect is 21.38 ng/mL in the presence of 1 ng/mL of rhTGF-beta 2, corresponding to a specific activity is 4.677×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit TGF-beta 2 activity on HT-2 mouse T cells. The ED50 for this effect is 21.38 ng/mL in the presence of 1 ng/mL of rhTGF-beta 2, corresponding to a specific activity is 4.677×104 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    A0A0R4J097 (G23-T785)

  • Gene ID
    21814  [NCBI]
  • Molecular Construction
    • N-term
    • TGFBR3 (G23-T785)
      Accession # A0A0R4J097
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    TGFBR3; TGF-Beta Receptor Type III; Transforming Growth Factor Beta Receptor 3; TGF-Beta Receptor Type 3; Betaglycan; Betaglycan Proteoglycan; Transforming Growth Factor Beta Receptor III; TGFR-3; Transforming Growth Factor Beta Receptor Type 3; Transform

  • AA Sequence

    GPEPSTRCELSPISASHPVQALMESFTVLSGCASRGTTGLPREVHILNLRSTDQGLGQPQREVTLHLNPIASVHTHHKPVVFLLNSPQPLVWHVKTERLAAGVPRLFLVSEGSVVQFSSGNFSLTAETEERSFPQENEHLLHWAQKEYGAVTSFTELKIARNIYIKVGEDQVFPPTCNIGKNFLSLNYLAEYLQPKAAEGCVLASQPHEKEVHIIELISPNSNPYSTFQVDIIIDIRPAREDPEVVKNLVLILKCKKSVNWVIKSFDVKGNLKVIAPDSIGFGKESERSMTVTKLVRNDIPSTQENLMKWALDNGYSPVTSYTIAPVANRFHLRLENNEEMRDEEVHTIPPELRILLGPDHLPALDSPSFQGEIPNGGFPFPFPDIPRRGWKEGEDRIPRPKEPIIPRVQLLPDHREPEEVQGGVNIALSVKCDNEKMVVAVDKDSFQTNGYSGMELTLLDPSCKAKMNGTHFVLESPLNGCGTRHRRSAPDGVVYYNSIVVQAPSPGDSSGWPDGYEDLESGDNGFPGDTDEGETAPLSRAGVVVFNCSLRQLRSPSGFQDQLDGNATFNMELYNTDLFLVPSPGVFSVAENEHVYVEVSVTKADQDLGFAIQTCFISPYSNPDRMSDYTIIENICPKDDSVKFYSSKRVHFPIPHAEVDKKRFSFVFKSVFNTSLLFLHCELTLCSRKKGSQKLPKCVTPDDACTSLDATMIWTMMQNKKTFTKPLAVVLQVDYKENVPNMKESSPVPPPPQIFHGLDTLT

  • Predicted Molecular Mass

    86.3 kDa

  • Molecular Weight

    Approximately 90-125 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit TGF-beta 2 activity on HT-2 mouse T cells. The ED50 for this effect is 21.38 ng/mL in the presence of 1 ng/mL of rhTGF-beta 2, corresponding to a specific activity is 4.677×104 units/mg.

  • TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His)
  • Experimental Validation Results for TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His)
  • TGFBR3/TGF-beta RIII Protein, Mouse (HEK293, His) Product Image