Tissue Factor Protein, Rabbit (HEK293, His)

Tissue Factor Protein initiates blood coagulation by binding to coagulation factors. It activates clotting cascades and interacts with cells to promote thrombosis and inflammation. Understanding Tissue Factor Protein's functions is crucial for developing treatments for cardiovascular diseases and blood disorders. Tissue Factor Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived Tissue Factor protein, expressed by HEK293 , with C-His labeled tag. The total length of Tissue Factor Protein, Rabbit (HEK293, His) is 218 a.a., with molecular weight of ~26.4 kDa.

Tissue Factor (TF) serves as the initiator of blood coagulation, creating a complex with circulating factor VII or VIIa. This [TF:VIIa] complex, through specific limited proteolysis, activates factors IX or X. In normal hemostasis, TF plays a pivotal role by instigating the assembly and propagation of the coagulation protease cascade on the cell surface. Furthermore, TF interacts with HSPE, and this interaction, hindered by heparin, facilitates the generation of activated factor X, ultimately contributing to the activation of coagulation in the presence of activated factor VII. This underscores the intricate involvement of TF in the regulatory processes of blood clotting.

Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC₅₀ is 5.368 μg/mL, as measured under the described conditions.

Rabbit

HEK293

C-His

P24055/NP_001075760.1 (A33-M250)

100009127  [NCBI]

ADTTGRAYNLTWKSTNFKTILEWEPKSIDHVYTVQISTRLENWKSKCFLTAETECDLTDEVVKDVGQTYMARVLSYPARNGNTTGFPEEPPFRNSPEFTPYLDTNLGQPTIQSFEQVGTKLNVTVQDARTLVRRNGTFLSLRAVFGKDLNYTLYYWRASSTGKKTATTNTNEFLIDVDKGENYCFSVQAVIPSRKRKQRSPESLTECTSREQGRAREM

Approximately 35-55 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.