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TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)

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Based on 1 Customer Validation

TL1A/TNFSF15 Protein, the receptor for TNFRSF25 and TNFRSF6B, activates NF-kappa-B and promotes caspase activation, leading to apoptosis. It also inhibits vascular endothelial growth and angiogenesis in vitro. Operating as a homotrimer, it plays a crucial role in diverse cellular processes, including immune response and apoptosis regulation. TL1A/TNFSF15 Trimer Protein, Human (HEK293, His) is the recombinant human-derived TL1A/TNFSF15 Trimer protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

TL1A/TNFSF15 Protein, the receptor for TNFRSF25 and TNFRSF6B, activates NF-kappa-B and promotes caspase activation, leading to apoptosis. It also inhibits vascular endothelial growth and angiogenesis in vitro. Operating as a homotrimer, it plays a crucial role in diverse cellular processes, including immune response and apoptosis regulation. TL1A/TNFSF15 Trimer Protein, Human (HEK293, His) is the recombinant human-derived TL1A/TNFSF15 Trimer protein, expressed by HEK293 , with N-His labeled tag.

Background

TL1A/TNFSF15 Protein acts as the receptor for TNFRSF25 and TNFRSF6B, facilitating the activation of NF-kappa-B and promoting the activation of caspases, leading to apoptosis. Additionally, it exhibits inhibitory effects on vascular endothelial growth and angiogenesis in vitro. The protein functions as a homotrimer, underscoring its role in diverse cellular processes, including immune response and apoptosis regulation.

Verified Bioactivity

Measured by its ability to induce apoptosis of TF-1 human erythroleukemic cells. The ED50 for this effect is 3.851 ng/mL, corresponding to a specific activity is 2.596×105 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce apoptosis of TF-1 human erythroleukemic cells. The ED50 for this effect is 3.851 ng/mL, corresponding to a specific activity is 2.596×105 units/mg.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His) on AHC2 biosensor, can bind Duvakitug (HY-P990006) with an affinity constant of 4.777E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-6*His
  • Accession

    O95150-1 (D91-L251)

  • Gene ID
    9966
  • Molecular Construction
    • N-term
    • 6*His
    • TL1A (D91-L251)
      Accession # O95150-1
    • C-term
  • Protein Length

    Full Length of THD Domain

  • Synonyms

    TNFSF15; TL1A; TNF Superfamily Member 15; Vascular Endothelial Growth Inhibitor-192A; Vascular Endothelial Cell Growth Inhibitor; Vascular Endothelial Growth Inhibitor; TNF Ligand-Related Molecule 1; TNF Superfamily Ligand TL1A; VEGI; MGC129934; TL1; MGC1

  • AA Sequence

    DGDKPRAHLTVVRQTPTQHFKNQFPALHWEHELGLAFTKNRMNYTNKFLLIPESGDYFIYSQVTFRGMTSECSEIRQAGRPNKPDSITVVITKVTDSYPEPTQLLMGTKSVCEVGSNWFQPIYLGAMFSLQEGDKLMVNVSDISLVDYTKEDKTFFGAFLL

  • Predicted Molecular Mass

    18.4 kDa

  • Molecular Weight

    Approximately 20-26 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce apoptosis of TF-1 human erythroleukemic cells. The ED50 for this effect is 3.851 ng/mL, corresponding to a specific activity is 2.596×105 units/mg.

  • 1/16

    MedChemExpress Validation
     Loaded TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His) on AHC2 biosensor, can bind Duvakitug (HY-P990006) with an affinity constant of 4.777E-10 M as determined in BLI assay.

  • TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • Experimental Validation Results for TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His)
  • TL1A/TNFSF15 Protein, Human (161a.a, HEK293, His) Product Image