TPBG/5T4 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This means that TPBG/5T4 plays a crucial role in regulating the complex Wnt/β-catenin signaling pathway and exerts a regulatory influence on cellular responses related to this pathway. TPBG/5T4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TPBG/5T4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization. This means that TPBG/5T4 plays a crucial role in regulating the complex Wnt/β-catenin signaling pathway and exerts a regulatory influence on cellular responses related to this pathway. TPBG/5T4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TPBG/5T4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
The TPBG/5T4 protein appears to serve as an inhibitor of Wnt/beta-catenin signaling, potentially achieved through indirect interaction with LRP6, thereby impeding Wnt3a-dependent LRP6 internalization. This implies a crucial role for TPBG/5T4 in modulating the intricate Wnt/beta-catenin signaling pathway, exerting regulatory influence on cellular responses associated with this pathway. Further exploration of the specific molecular mechanisms governing the interaction between TPBG/5T4 and LRP6, as well as its impact on Wnt3a-dependent LRP6 internalization, could yield valuable insights into the functional significance of TPBG/5T4 in shaping Wnt/beta-catenin signaling dynamics and its potential implications for various cellular processes.
Verified Bioactivity
1.Immobilized Biotinylated Human TPBG, His Tag at 0.5 μg/mL (100 μL/Well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for AntiTPBG Antibody, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.
2.Immobilized Biotinylated Human TPBG, His Tag at 1 μg/mL (100 μL/Well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for AntiTPBG Antibody, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
Q13641 (S32-S355)
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Molecular Construction
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N-term
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TLR8 (S32-S355)
Accession # Q13641 -
His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
TPBG; 5T4 Oncofetal Antigen; Trophoblast Glycoprotein; 5T4-AG; 5T4; WAIF1; 5T4 Oncofetal Trophoblast Glycoprotein; M6P1; Wnt-Activated Inhibitory Factor 1; 5T4AG; 5T4 Oncotrophoblast Glycoprotein
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AA Sequence
SSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS
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Predicted Molecular Mass
38.2 kDa
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Molecular Weight
Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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