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TPBG/5T4 Protein, Mouse (HEK293, His)

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TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization.This suggests that TPBG/5T4 plays a key role in regulating the complex Wnt/β-catenin signaling pathway and influencing cellular responses related to this pathway.TPBG/5T4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPBG/5T4 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • TPBG/5T4 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Top Publications Citing

Use of MCE Products

Biological Activity

Description

TPBG/5T4 protein acts as an inhibitor of Wnt/β-catenin signaling, possibly by interacting indirectly with LRP6 and hindering Wnt3a-dependent LRP6 internalization.This suggests that TPBG/5T4 plays a key role in regulating the complex Wnt/β-catenin signaling pathway and influencing cellular responses related to this pathway.TPBG/5T4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPBG/5T4 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The TPBG/5T4 protein appears to operate as an inhibitor of Wnt/beta-catenin signaling, potentially exerting its inhibitory effects by indirectly interacting with LRP6 and impeding Wnt3a-dependent LRP6 internalization. This suggests that TPBG/5T4 may play a critical role in modulating the intricate Wnt/beta-catenin signaling pathway, influencing cellular responses and functions associated with this pathway. A comprehensive investigation into the specific molecular mechanisms underlying the interaction between TPBG/5T4 and LRP6, as well as its impact on Wnt3a-dependent LRP6 internalization, could provide valuable insights into the functional significance of TPBG/5T4 in the regulation of Wnt/beta-catenin signaling and its potential implications for cellular processes.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9Z0L0 (S32-S361)

  • Gene ID
    21983  [NCBI]
  • Molecular Construction
    • N-term
    • TLR8 (S32-S361)
      Accession # Q9Z0L0
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    TPBG; 5T4 Oncofetal Antigen; Trophoblast Glycoprotein; 5T4-AG; 5T4; WAIF1; 5T4 Oncofetal Trophoblast Glycoprotein; M6P1; Wnt-Activated Inhibitory Factor 1; 5T4AG; 5T4 Oncotrophoblast Glycoprotein

  • AA Sequence

    SAPSSSVPSSSTSPAAFLASGSAQPPPAERCPAACECSEAARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLEALNLSGNHLKEVCAGAFEHLPGLRRLDLSHNPLTNLSAFAFAGSNASVSAPSPLEELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALRSGLALRGLTRLELASNHFLFLPRDLLAQLPSLRYLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLHNSTLAEWHGLAHVKVFLDNNPWVCDCYMADMVAWLKETEVVPDKARLTCAFPEKMRNRGLLDLNSSDLDCDAVLPQSLQTS

  • Predicted Molecular Mass

    36.6 kDa

  • Molecular Weight

    Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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