TPO/Thrombopoietin Protein, Mouse (HEK293, N-His)
Based on 1 Customer Validation
TPO/Thrombopoietin Protein, a lineage-specific cytokine, crucially regulates megakaryocyte proliferation and maturation, acting in the late stages of development from committed progenitor cells. Recognized as a major physiological regulator, TPO significantly influences circulating platelets, emphasizing its importance in the intricate process of platelet formation. TPO/Thrombopoietin Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived TPO/Thrombopoietin protein, expressed by HEK293 , with N-6*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TPO/Thrombopoietin Protein, a lineage-specific cytokine, crucially regulates megakaryocyte proliferation and maturation, acting in the late stages of development from committed progenitor cells. Recognized as a major physiological regulator, TPO significantly influences circulating platelets, emphasizing its importance in the intricate process of platelet formation. TPO/Thrombopoietin Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived TPO/Thrombopoietin protein, expressed by HEK293 , with N-6*His labeled tag.
Background
Thrombopoietin (TPO), a lineage-specific cytokine, plays a crucial role in regulating the proliferation and maturation of megakaryocytes, acting at a late stage of their development from committed progenitor cells. Functioning as a key factor in megakaryopoiesis, TPO is recognized as a major physiological regulator of circulating platelets. Its influence on the later stages of megakaryocyte development underscores its significance in the intricate process of platelet formation.
Verified Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.9757 ng/mL, corresponding to a specific activity is 1.25×106 units/mg.
MCE Validation Data
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Mouse
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Source HEK293
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Tag N-6*His
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Accession
P40226-1 (S22-T356)
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Molecular Construction
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N-term
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6*His
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TPO/Thrombopoietin (S22-T356)
Accession # P40226-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
THPO; Prepro-Thrombopoietin; Prev. MGDF; MPL Ligand; Myeloproliferative Leukemia Virus Oncogene Ligand; ML; Megakaryocyte Growth And Development Factor; Thrombopoietin Nirs Variant 3; Megakaryocyte Colony-Stimulating Factor; Thrombopoietin Nirs Variant 2;
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AA Sequence
SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET
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Predicted Molecular Mass
36.4 kDa
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Molecular Weight
Approximately 65-105 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 2 mM EDTA, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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