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  5. TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His)

TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His)

Cat. No.: HY-P78045
COA Handling Instructions

Thyroid Peroxidase (TPO) orchestrates iodination and coupling within thyroglobulin, leading to the synthesis of thyroid hormones T(3) and T(4). TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPO/Thyroid Peroxidase protein, expressed by HEK293 , with C-His labeled tag. The total length of TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His) is 803 a.a., with molecular weight of 100-115 kDa.

For research use only. We do not sell to patients.

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Description

Thyroid Peroxidase (TPO) orchestrates iodination and coupling within thyroglobulin, leading to the synthesis of thyroid hormones T(3) and T(4). TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His) is the recombinant mouse-derived TPO/Thyroid Peroxidase protein, expressed by HEK293 , with C-His labeled tag. The total length of TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His) is 803 a.a., with molecular weight of 100-115 kDa.
Background

TPO, or Thyroid Peroxidase, orchestrates the iodination and coupling processes of the hormonogenic tyrosines within thyroglobulin, culminating in the synthesis of thyroid hormones T(3) and T(4).
Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

P35419 (G32-R834)
Gene ID

22018  [NCBI]
Molecular Construction
N-term
TPO (G32-R834)
Accession # P35419
His
C-term
Synonyms
TPO; THPO; MGC163194; MGDF; MKCSF; ML; MPLLG; THCYT1; MGDFC-mpl ligand; MK-CSF; MPL ligand; Thrombopoietin; TPOMKCSF
AA Sequence

GKTMKSHVISAVETSQLMVDHAVYNTMKRNLKKREVLSPAQLLSFFKLPESTSGAISRAAEIMETSIQVMKREQSQFSTDALSADILGTIANLSGCLPFMLPPRCPDTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPKGWNPNFLYHGFPLPPVREVTRHLIQVSNEAVTEDDQYSDFLPVWGQYIDHDIALTPQSTSTAAFWGGVDCQLTCENQNPCFPIQLPSNSSGTTACLPFYRSSAACGTGDQGALFGNLSAANPRQQMNGLTSFLDASTVYGSSPGVEKQLRNWSSSAGLLRVNTLHLDAGRAYLPFATAACAPEPGTPRTNRTPCFLAGDGRASEVPALAAVHTLWLREHNRLASAFKAINKHWSANTAYQEARKVVGALHQIITMRDYIPKILGPDAFRQYVGPYEGYNPTVNPTVSNIFSTAAFRFGHATVHPLVRRLNTDFQEHTELPRLQLRDVFFRPWRLIQEGGLDPIVRGLLARAAKLQVQGQLMNEELTERLFVLSNVGTLDLASLNLQRGRDHGLPDYNEWREFCGLSRLETPAELNKAIANRSMVNKIMDLYKHADNIDVWLGGLAEKFLPGARTGPLFACIIGKQMKALRDGDRFWWENTNVFTDAQRQELEKHSLPRVICDNTGLTRVPVDAFRIGKFPQDFESCEDIPSMDLELWRETFPQDDKCVFPEEVDNGNFVHCEESGKLVLVYSCFHGYKLQGQEQVTCTQKGWDSEPPVCKDVNECADLTHPPCHPSAQCKNTKGSFQCVCTDPYVLGEDEKTCIDSGRLPR

Molecular Weight

100-115 kDa
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

TPO/Thyroid Peroxidase Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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