1. Recombinant Proteins
  2. Others
  3. Transferrin Protein, Mouse (HEK293, His)

Transferrin Protein, Mouse (HEK293, His)

Cat. No.: HY-P73453
COA Handling Instructions

Transferrin is an important iron-binding transport protein that combines two Fe(3+) ions with an anion, usually bicarbonate. Its main function is to transport iron from the site of absorption to the site of storage and utilization. Transferrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Transferrin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Transferrin Protein, Mouse (HEK293, His) is 678 a.a., with molecular weight of approximately 85 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $35 In-stock
10 μg $60 In-stock
50 μg $170 In-stock
100 μg $290 In-stock
250 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Transferrin is an important iron-binding transport protein that combines two Fe(3+) ions with an anion, usually bicarbonate. Its main function is to transport iron from the site of absorption to the site of storage and utilization. Transferrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Transferrin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Transferrin Protein, Mouse (HEK293, His) is 678 a.a., with molecular weight of approximately 85 kDa.

Background

Transferrin, a pivotal iron-binding transport protein, exhibits the capacity to bind two Fe(3+) ions in conjunction with an anion, typically bicarbonate. Its primary responsibility lies in facilitating the transport of iron from absorption sites and heme degradation to locations designated for storage and utilization within the body. Beyond its fundamental role in iron homeostasis, serum transferrin may additionally play a role in promoting cell proliferation. Structured as a monomer, transferrin's multifaceted functions underscore its significance in orchestrating the intricate balance of iron distribution and cellular processes essential for overall physiological well-being.

Biological Activity

Measured in a cell proliferation assay using A549 human non small cell lung cancer cell. The ED50 for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.

  • Measured in a cell proliferation assay using A549 human non small cell lung cancer cell. The ED50 for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.
Species

Mouse

Source

HEK293

Tag

C-10*His

Accession

Q921I1 (V20-H697)

Gene ID

22041  [NCBI]

Synonyms
Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF; TRF
AA Sequence

VPDKTVKWCAVSEHENTKCISFRDHMKTVLPPDGPRLACVKKTSYPDCIKAISASEADAMTLDGGWVYDAGLTPNNLKPVAAEFYGSVEHPQTYYYAVAVVKKGTDFQLNQLEGKKSCHTGLGRSAGWVIPIGLLFCKLSEPRSPLEKAVSSFFSGSCVPCADPVAFPKLCQLCPGCGCSSTQPFFGYVGAFKCLKDGGGDVAFVKHTTIFEVLPEKADRDQYELLCLDNTRKPVDQYEDCYLARIPSHAVVARKNNGKEDLIWEILKVAQEHFGKGKSKDFQLFSSPLGKDLLFKDSAFGLLRVPPRMDYRLYLGHNYVTAIRNQQEGVCPEGSIDNSPVKWCALSHLERTKCDEWSIISEGKIECESAETTEDCIEKIVNGEADAMTLDGGHAYIAGQCGLVPVMAEYYESSNCAIPSQQGIFPKGYYAVAVVKASDTSITWNNLKGKKSCHTGVDRTAGWNIPMGMLYNRINHCKFDEFFSQGCAPGYEKNSTLCDLCIGPLKCAPNNKEEYNGYTGAFRCLVEKGDVAFVKHQTVLDNTEGKNPAEWAKNLKQEDFELLCPDGTRKPVKDFASCHLAQAPNHVVVSRKEKAARVKAVLTSQETLFGGSDCTGNFCLFKSTTKDLLFRDDTKCFVKLPEGTTPEKYLGAEYMQSVGNMRKCSTSRLLEACTFHKH

Molecular Weight

approximately 85 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Transferrin Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Transferrin Protein, Mouse (HEK293, His)
Cat. No.:
HY-P73453
Quantity:
MCE Japan Authorized Agent: