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Transferrin Protein, Mouse (HEK293, His)

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Transferrin is an important iron-binding transport protein that combines two Fe(3+) ions with an anion, usually bicarbonate.Its main function is to transport iron from the site of absorption to the site of storage and utilization.Transferrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Transferrin protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Transferrin Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Transferrin Protein, Mouse (HEK293, His)
  • Transferrin Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Transferrin is an important iron-binding transport protein that combines two Fe(3+) ions with an anion, usually bicarbonate.Its main function is to transport iron from the site of absorption to the site of storage and utilization.Transferrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Transferrin protein, expressed by HEK293 , with C-10*His labeled tag.

Background

Transferrin, a pivotal iron-binding transport protein, exhibits the capacity to bind two Fe(3+) ions in conjunction with an anion, typically bicarbonate. Its primary responsibility lies in facilitating the transport of iron from absorption sites and heme degradation to locations designated for storage and utilization within the body. Beyond its fundamental role in iron homeostasis, serum transferrin may additionally play a role in promoting cell proliferation. Structured as a monomer, transferrin's multifaceted functions underscore its significance in orchestrating the intricate balance of iron distribution and cellular processes essential for overall physiological well-being.

Verified Bioactivity

1. Measured by its ability to inhibit proliferation of A549 cells. The ED50 for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.
2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 15-150ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q921I1 (V20-H697)

  • Gene ID
    22041  [NCBI]
  • Molecular Construction
    • N-term
    • Transferrin (V20-H697)
      Accession # Q921I1
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    TF; Epididymis Secretory Sperm Binding Protein Li 71p; Transferrin; CDNA FLJ54029, Highly Similar To Serotransferrin; Serotransferrin; Transferrin, Isoform CRA_c; Beta-1 Metal-Binding Globulin; TF Protein; Siderophilin; HEL-S-71p; PRO1557; TFQTL1; PRO2086

  • AA Sequence

    VPDKTVKWCAVSEHENTKCISFRDHMKTVLPPDGPRLACVKKTSYPDCIKAISASEADAMTLDGGWVYDAGLTPNNLKPVAAEFYGSVEHPQTYYYAVAVVKKGTDFQLNQLEGKKSCHTGLGRSAGWVIPIGLLFCKLSEPRSPLEKAVSSFFSGSCVPCADPVAFPKLCQLCPGCGCSSTQPFFGYVGAFKCLKDGGGDVAFVKHTTIFEVLPEKADRDQYELLCLDNTRKPVDQYEDCYLARIPSHAVVARKNNGKEDLIWEILKVAQEHFGKGKSKDFQLFSSPLGKDLLFKDSAFGLLRVPPRMDYRLYLGHNYVTAIRNQQEGVCPEGSIDNSPVKWCALSHLERTKCDEWSIISEGKIECESAETTEDCIEKIVNGEADAMTLDGGHAYIAGQCGLVPVMAEYYESSNCAIPSQQGIFPKGYYAVAVVKASDTSITWNNLKGKKSCHTGVDRTAGWNIPMGMLYNRINHCKFDEFFSQGCAPGYEKNSTLCDLCIGPLKCAPNNKEEYNGYTGAFRCLVEKGDVAFVKHQTVLDNTEGKNPAEWAKNLKQEDFELLCPDGTRKPVKDFASCHLAQAPNHVVVSRKEKAARVKAVLTSQETLFGGSDCTGNFCLFKSTTKDLLFRDDTKCFVKLPEGTTPEKYLGAEYMQSVGNMRKCSTSRLLEACTFHKH

  • Predicted Molecular Mass

    76.3 kDa

  • Molecular Weight

    Approximately 61-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit proliferation of A549 cells. The ED50 for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.

  • Transferrin Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Transferrin Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Transferrin Protein, Mouse (HEK293, His)
  • Transferrin Protein, Mouse (HEK293, His) Product Image