1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. treS Protein, Mycobacterium tuberculosis

treS Protein, Mycobacterium tuberculosis is the recombinant treS, expressed by E. coli , with tag Free labeled tag. ,

For research use only. We do not sell to patients.

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Description

treS Protein, Mycobacterium tuberculosis is the recombinant treS, expressed by E. coli , with tag Free labeled tag. ,

Background

The treS protein is an enzyme that plays a crucial role in carbohydrate metabolism by catalyzing the reversible interconversion of maltose and alpha,alpha-trehalose through transglucosylation. This enzymatic activity is involved in the biosynthesis and breakdown of trehalose, a disaccharide composed of two glucose molecules linked by an alpha-1,1-glycosidic bond. Trehalose serves as a versatile and protective sugar in various organisms, playing roles in stress response, energy storage, and cellular protection. The reversible action of treS facilitates the dynamic regulation of trehalose levels based on cellular requirements. Understanding the functions of treS provides insights into the intricate network of carbohydrate metabolism and the versatile roles of trehalose in cellular processes.

Species

Others

Source

E. coli

Tag

Tag Free

Accession

P9WQ19 (M1-L601)

Gene ID

45424092

Protein Length

Full Length

Synonyms
Trehalose synthase/amylase TreS; Maltose alpha-D-glucosyltransferase; MTase; treS; Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv); Glycosidase; Hydrolase; Isomerase; 3.2.1.1
AA Sequence

MNEAEHSVEHPPVQGSHVEGGVVEHPDAKDFGSAAALPADPTWFKHAVFYEVLVRAFFDASADGSGDLRGLIDRLDYLQWLGIDCIWLPPFYDSPLRDGGYDIRDFYKVLPEFGTVDDFVALVDAAHRRGIRIITDLVMNHTSESHPWFQESRRDPDGPYGDYYVWSDTSERYTDARIIFVDTEESNWSFDPVRRQFYWHRFFSHQPDLNYDNPAVQEAMIDVIRFWLGLGIDGFRLDAVPYLFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPGDVVEYFGDPNTGGDECHMAFHFPLMPRIFMAVRRESRFPISEIIAQTPPIPDMAQWGIFLRNHDELTLEMVTDEERDYMYAEYAKDPRMKANVGIRRRLAPLLDNDRNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRIPMQWTPDRNAGFSTANPGRLYLPPSQDPVYGYQAVNVEAQRDTSTSLLNFTRTMLAVRRRHPAFAVGAFQELGGSNPSVLAYVRQVAGDDGDTVLCVNNLSRFPQPIELDLQQWTNYTPVELTGHVEFPRIGQVPYLLTLPGHGFYWFQLTTHEVGAPPTCGGERRL

Predicted Molecular Mass
68.8 kDa
Purity

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl , pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

treS Protein, Mycobacterium tuberculosis Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
treS Protein, Mycobacterium tuberculosis
Cat. No.:
HY-P703441
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