TrkA Protein, Mouse (HEK293, His)

TrkA protein is a key receptor tyrosine kinase that guides the development and maturation of the nervous system and affects neuronal proliferation, differentiation, and survival. TrkA serves as a high-affinity receptor for NGF (its primary ligand) and also responds to NTF3/neurotropin-3. TrkA Protein, Mouse (HEK293, His) is the recombinant mouse-derived TrkA protein, expressed by HEK293 , with C-His, C-8*His labeled tag. The total length of TrkA Protein, Mouse (HEK293, His) is 387 a.a., with molecular weight of ~55-125 kDa.

TrkA Protein is a receptor tyrosine kinase that plays a crucial role in the development and maturation of the central and peripheral nervous systems by regulating the proliferation, differentiation, and survival of sympathetic and sensory neurons. It serves as a high-affinity receptor for NGF, which is its primary ligand, and can also be activated by NTF3/neurotrophin-3. While NTF3 only supports axonal extension through NTRK1, it does not have an impact on neuron survival. Upon binding to dimeric NGF ligands, TrkA undergoes homodimerization, autophosphorylation, and activation. This activation leads to the recruitment, phosphorylation, and/or activation of various downstream effectors such as SHC1, FRS2, SH2B1, SH2B2, and PLCG1, which in turn regulate distinct overlapping signaling pathways that drive cell survival and differentiation. Specifically, through SHC1 and FRS2, TrkA activates a GRB2-Ras-MAPK cascade that controls cell differentiation and survival. Additionally, through PLCG1, TrkA regulates NF-Kappa-B activation and the transcription of genes involved in cell survival. Moreover, TrkA also controls a Ras-PI3 kinase-AKT1 signaling cascade, mediated by SHC1 and SH2B1, which further contributes to cell survival. In the absence of ligand and activation, TrkA may promote cell death, making the survival of neurons dependent on trophic factors.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-8*His

Q3UFB7/NP_001028296.1 (A34-G420)

18211  [NCBI]

ASCREVCCPVGPSGLRCTRAGSLDTLRGLRGAGNLTELYVENQQHLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFRFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALFWLQRWEQEGLCGVHTQTLHDSGPGDQFLPLGHNTSCGVPTVKIQMPNDSVEVGDDVFLQCQVEGLALQQADWILTELEGAATVKKFGDLPSLGLILVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGLAVEQHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASVMAAFMDNPFEFNPEDPIPVSFSPVDGNSTSRDPVEKKDETPFG

Approximately 55-125 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.