UBA5 Protein, Human (His)

The UBA5 protein activates UFM1 in ufmylation, linking the C-terminal glycine residue of UFM1 to a cysteine residue in E1. UBA5 Protein, Human (His) is the recombinant human-derived UBA5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of UBA5 Protein, Human (His) is 404 a.a., with molecular weight of 14 & 40-60 kDa, respectively.

UBA5, functioning as an E1-like enzyme, plays a pivotal role in the initiation of ufmylation, a post-translational modification process. The activation of UFM1 begins with the adenylation of its C-terminal glycine residue by UBA5 in conjunction with ATP. This results in the formation of a UFM1-E1 thioester and free AMP. Notably, UBA5 achieves this through a trans-binding mechanism, where UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. This trans-binding not only facilitates the stabilization of the UBA5 homodimer but also enhances ATP-binding. Subsequently, UFM1 is transferred from UBA5 to the E2-like enzyme UFC1, again employing a trans mechanism. Ufmylation, orchestrated by UBA5, emerges as a critical process involved in reticulophagy (ER-phagy) triggered by endoplasmic reticulum stress. Moreover, Ufmylation proves indispensable for the erythroid differentiation of both megakaryocytes and erythrocytes. UBA5 exists as a homodimer, and this homodimerization is essential for UFM1 activation. UBA5 also engages in specific interactions with UFM1, GABARAPL2, GABARAP, GABARAPL1, and UFC1, underscoring its intricate involvement in diverse cellular processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9GZZ9 (M1-M404)

79876  [NCBI]

MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

14&40-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 10% Glycerol, pH 8.0 .

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.