UBE2I Protein, Human (GST)
Based on 1 Customer Validation
The UBE2I protein is a key player in protein sumoylation, accepting the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. UBE2I cooperates with E3 ligases such as RANBP2, CBX4, and ZNF451 to catalyze the covalent attachment of these SUMO proteins to target proteins, including key substrates FOXL2 and KAT5. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli , with N-GST labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The UBE2I protein is a key player in protein sumoylation, accepting the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. UBE2I cooperates with E3 ligases such as RANBP2, CBX4, and ZNF451 to catalyze the covalent attachment of these SUMO proteins to target proteins, including key substrates FOXL2 and KAT5. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli , with N-GST labeled tag.
UBE2I, a crucial player in the protein sumoylation process, demonstrates versatility by accepting ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Collaborating with various E3 ligases, such as RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins. This enzymatic activity extends to the formation of poly-SUMO chains, emphasizing its role in diverse cellular processes. UBE2I is essential for sumoylation events involving critical substrates like FOXL2 and KAT5, contributing to nuclear architecture and chromosome segregation. Furthermore, UBE2I-mediated sumoylation plays a pivotal role in specific protein modifications, including sumoylation of p53/TP53 at 'Lys-386' and ERCC6, the latter being indispensable for its transcription-coupled nucleotide excision repair activity.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-GST
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Accession
P63279 (M1-S158)
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Molecular Construction
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N-term
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GST
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UBE2I (M1-S158)
Accession # P63279 -
C-term
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Protein Length
Full Length
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Synonyms
UBE2I; Ubiquitin Conjugating Enzyme E2 I; UBC9; RING‑Type E3 SUMO Transferase UBC9; SUMO‑Conjugating Enzyme UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin‑Protein Ligase I; SUMO‑Protein Ligase; Ubiquitin‑Conjugating Enzyme E2I (
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AA Sequence
MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
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Predicted Molecular Mass
44.4 kDa
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Molecular Weight
Approximately 40 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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