ULK3 Protein, Human (sf9, His, GST)
Based on 1 Customer Validation
ULK3 Protein, Human (sf9, His, GST) is the recombinant human-derived ULK3, expressed by sf9 insect cells , with His, GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ULK3 Protein, Human (sf9, His, GST) is the recombinant human-derived ULK3, expressed by sf9 insect cells , with His, GST labeled tag. ,
Background
ULK3 is a serine/threonine protein kinase that regulates Sonic hedgehog (SHH) signaling and autophagy. In the absence of SHH ligand, ULK3 acts as a negative regulator of SHH signaling by interacting with SUFU, inactivating its kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2, and/or GLI3). In the presence of SHH, ULK3 dissociates from SUFU, undergoes autophosphorylation, and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. In vitro, ULK3 phosphorylates GLI2, as well as GLI1 and GLI3, though less efficiently. Additionally, ULK3 induces autophagy following cellular senescence, functioning as a regulator of autophagy.
Verified Bioactivity
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
Q6PHR2-1 (A2-Q472)
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Gene ID25989
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Protein Length
Full Length of Isoform-1
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Synonyms
ULK3; Unc-51 Like Kinase 3; Serine/Threonine-Protein Kinase ULK3; DKFZP434C131; FLJ90566; Non-Specific Serine/Threonine Protein Kinase; Unc-51-Like Kinase 3 (C. Elegans); Unc-51-Like Kinase 3
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AA Sequence
AGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGIRHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHERNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSMGVILYEALFGQPPFASRSFSELEEKIRSNRVIELPLRPLLSRDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMPSGESLGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSSSNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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