UMP-CMP kinase/CMPK1 Protein, Human (sf9, His, solution)
Based on 1 Customer Validation
The UMP-CMP kinase (CMPK1) protein utilizes ATP as a phosphate donor to catalyze the phosphorylation of pyrimidine nucleoside monophosphate, thereby playing a key role in cellular processes. This enzymatic activity is integral to the de novo biosynthesis of pyrimidine nucleotides and contributes to the synthesis of important cellular building blocks. UMP-CMP kinase/CMPK1 Protein, Human (sf9, His, solution) is the recombinant human-derived UMP-CMP kinase/CMPK1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The UMP-CMP kinase (CMPK1) protein utilizes ATP as a phosphate donor to catalyze the phosphorylation of pyrimidine nucleoside monophosphate, thereby playing a key role in cellular processes. This enzymatic activity is integral to the de novo biosynthesis of pyrimidine nucleotides and contributes to the synthesis of important cellular building blocks. UMP-CMP kinase/CMPK1 Protein, Human (sf9, His, solution) is the recombinant human-derived UMP-CMP kinase/CMPK1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
The UMP-CMP kinase, also known as CMPK1, is a crucial enzyme that catalyzes the phosphorylation of pyrimidine nucleoside monophosphates utilizing ATP as a phosphate donor. This enzymatic activity holds significance in de novo pyrimidine nucleotide biosynthesis, representing a key step in the synthesis of essential cellular components. CMPK1 exhibits a preference for uridine monophosphate (UMP) and cytidine monophosphate (CMP) as phosphate acceptors in this process. Additionally, CMPK1 displays broad nucleoside diphosphate kinase activity, emphasizing its versatility in catalyzing the transfer of phosphate groups between different nucleoside diphosphates. The multifaceted functions of CMPK1 underscore its pivotal role in cellular nucleotide metabolism and its potential implications for various cellular processes.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P30085-1 (M1-G196)
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Molecular Construction
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N-term
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His
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CMPK1 (M1-G196)
Accession # P30085-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
CMPK1; CK; Prev. CMPK; Cytidylate Kinase; UMP-CMP Kinase; UMP/CMPK; UMP/CMP Kinase; UMPK; Uridine Monophosphate/Cytidine Monophosphate Kinase; CMK; Nucleoside-Diphosphate Kinase; UMK; Deoxycytidylate Kinase; Cytidine Monophosphate Kinase; DCMP Kinase; Uri
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AA Sequence
MKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
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Predicted Molecular Mass
24.5 kDa
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Molecular Weight
Approximately 27 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM Nacl, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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