USF1 Protein, Human (His)
The USF1 protein is a transcription factor that binds symmetric E-box DNA (5'-CACGTG-3') in viral and cellular promoters. Efficient DNA binding requires dimerization, typically using bHLH proteins. USF1 Protein, Human (His) is the recombinant human-derived USF1 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The USF1 protein is a transcription factor that binds symmetric E-box DNA (5'-CACGTG-3') in viral and cellular promoters. Efficient DNA binding requires dimerization, typically using bHLH proteins. USF1 Protein, Human (His) is the recombinant human-derived USF1 protein, expressed by E. coli , with N-6*His labeled tag.
The USF1 protein functions as a transcription factor, binding to symmetrical DNA sequences known as E-boxes (5'-CACGTG-3'), which are prevalent in a diverse array of viral and cellular promoters. Effective DNA binding necessitates dimerization, typically with another basic helix-loop-helix (bHLH) protein. USF1 engages in DNA binding both as a homodimer and as a heterodimer in association with USF2. Notably, USF1 also interacts with the varicella-zoster virus IE62 protein, indicating its involvement in molecular interactions with viral components. This versatile binding capacity and interaction profile highlight the regulatory role of USF1 in transcriptional processes across various cellular and viral contexts.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P22415 (G3-N310)
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Molecular Construction
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N-term
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6*His
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USF1 (G3-N310)
Accession # P22415 -
C-term
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Protein Length
Partial
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Synonyms
USF1; Upstream Transcription Factor 1; BHLHb11; Major Late Transcription Factor 1; Upstream Stimulatory Factor 1; MLTFI; UEF; Class B Basic Helix‑Loop‑Helix Protein 11; HYPLIP1; BHLHB11; FCHL1; FCHL; MLTF; USF
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AA Sequence
GQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
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Predicted Molecular Mass
37.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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