VCPIP1 Protein, Human (sf9, His)

The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (sf9, His) is the recombinant human-derived VCPIP1 protein, expressed by sf9 insect cells , with C-8*His labeled tag.

VCPIP1 Protein, a multifaceted deubiquitinating enzyme, plays essential roles in diverse cellular processes. It is intricately involved in DNA repair and the reassembly of the Golgi apparatus and endoplasmic reticulum post-mitosis, facilitating VCP-mediated Golgi stack reassembly and transitional endoplasmic reticulum (tER) formation. Additionally, VCPIP1 participates in the dissociation of the ternary complex containing STX5A, NSFL1C, and VCP. Moreover, following phosphorylation by ATM or ATR, VCPIP1 contributes to DNA repair by catalyzing the deubiquitination of SPRTN, promoting SPRTN recruitment to chromatin and facilitating the proteolytic cleavage of covalent DNA-protein cross-links (DPCs). The enzyme further demonstrates its versatility by hydrolyzing 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. In the context of microbial infection, VCPIP1 regulates the duration of C.botulinum neurotoxin type A (BoNT/A) intoxication by catalyzing the deubiquitination of Botulinum neurotoxin A light chain (LC), thereby preventing LC degradation by the proteasome and accelerating botulinum neurotoxin intoxication in patients. This highlights the diverse and crucial functions of VCPIP1 in cellular homeostasis and response to external challenges.

Ubiquitin-Rhodamine 110 (Ub-Rho110) is a fluorogenic rhodamine-based substrate. Ub-Rho110 is an ubiquitin substrate whose C-terminal derivatives are Rho110. While rhodamine is in the Ub-Rho110 moiety, it is di substituted thereby quenching the intrinsic fluorescence. However, mono-substituted rhodamine, which exhibits intense fluorescence, is released by the DUBs, thus allowing the real-time monitoring of the sensitivity or activity of DUBs by monitoring the 535 nm emission wavelengths after using 485 nm excitation. The increase in fluorescence is proportional to the DUB activity.

Human

Sf9 insect cells

C-8*His

Q96JH7 (M1-S1222)

80124

Full Length

MSQPPPPPPPLPPPPPPPEAPQTPSSLASAAASGGLLKRRDRRILSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNYSFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDGDRTNSRSTGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSNVYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQESESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAEGGQSAAAHSAHTVKQEDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSYAKGLPHMFQQGGVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMDRHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATRSKAQRGNSVEELEEMDSQDAEMTNTTEPMDHS

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.