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VCPIP1 Protein, Human (sf9, His)

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The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (sf9, His) is the recombinant human-derived VCPIP1 protein, expressed by sf9 insect cells , with C-8*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (sf9, His) is the recombinant human-derived VCPIP1 protein, expressed by sf9 insect cells , with C-8*His labeled tag.

Background

VCPIP1 Protein, a multifaceted deubiquitinating enzyme, plays essential roles in diverse cellular processes. It is intricately involved in DNA repair and the reassembly of the Golgi apparatus and endoplasmic reticulum post-mitosis, facilitating VCP-mediated Golgi stack reassembly and transitional endoplasmic reticulum (tER) formation. Additionally, VCPIP1 participates in the dissociation of the ternary complex containing STX5A, NSFL1C, and VCP. Moreover, following phosphorylation by ATM or ATR, VCPIP1 contributes to DNA repair by catalyzing the deubiquitination of SPRTN, promoting SPRTN recruitment to chromatin and facilitating the proteolytic cleavage of covalent DNA-protein cross-links (DPCs). The enzyme further demonstrates its versatility by hydrolyzing 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. In the context of microbial infection, VCPIP1 regulates the duration of C.botulinum neurotoxin type A (BoNT/A) intoxication by catalyzing the deubiquitination of Botulinum neurotoxin A light chain (LC), thereby preventing LC degradation by the proteasome and accelerating botulinum neurotoxin intoxication in patients. This highlights the diverse and crucial functions of VCPIP1 in cellular homeostasis and response to external challenges.

Verified Bioactivity

Ubiquitin-Rhodamine 110 (Ub-Rho110) is a fluorogenic rhodamine-based substrate. Ub-Rho110 is an ubiquitin substrate whose C-terminal derivatives are Rho110. While rhodamine is in the Ub-Rho110 moiety, it is di substituted thereby quenching the intrinsic fluorescence. However, mono-substituted rhodamine, which exhibits intense fluorescence, is released by the DUBs, thus allowing the real-time monitoring of the sensitivity or activity of DUBs by monitoring the 535 nm emission wavelengths after using 485 nm excitation. The increase in fluorescence is proportional to the DUB activity.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-8*His
  • Accession

    Q96JH7 (M1-S1222)

  • Gene ID
    80124
  • Molecular Construction
    • N-term
    • VCPIP1 (M1-S1222)
      Accession # Q96JH7
    • 8*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    VCPIP1; Valosin Containing Protein Interacting Protein 1; VCIP135; KIAA1850; DUBA3; Valosin-Containing Protein P97/P47 Complex-Interacting Protein P135; Valosin-Containing Protein P97/P47 Complex-Interacting Protein 1; VCP/P47 Complex-Interacting 135-KDa

  • AA Sequence

    MSQPPPPPPPLPPPPPPPEAPQTPSSLASAAASGGLLKRRDRRILSGSCPDPKCQARLFFPASGSVSIECTECGQRHEQQQLLGVEEVTDPDVVLHNLLRNALLGVTGAPKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELFDCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECLIPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFHDFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDSLSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPLVGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDKYLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAKKAVMDNRLHKCLLCGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNYSFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYLDGDRTNSRSTGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVYWFQYESDSSLNSNVYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKKNPDDYTPVNIDGAHAQRVGDVQGQESESQLPTKIILTGQKTKTLHKEELNMSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSAPATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREFNIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAEGGQSAAAHSAHTVKQEDIAVTGKLSSKELQEQAEKEMYSLCLLATLMGEDVWSYAKGLPHMFQQGGVFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMDRHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATRSKAQRGNSVEELEEMDSQDAEMTNTTEPMDHS

  • Predicted Molecular Mass

    136.3 KDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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