VE-Cadherin Protein, Mouse (HEK293, His)

VE-cadherin is a calcium-dependent glycoprotein critical for mediating cell adhesion and coordinating morphogenetic events during development. Lack of VE-cadherin in mice leads to uterine death due to increased endothelial cell apoptosis leading to vascular insufficiency. VE-Cadherin Protein, Mouse (HEK293, His) is the recombinant mouse-derived VE-Cadherin protein, expressed by HEK293 , with C-10*His labeled tag.

VE-Cadherin, a member of the cadherin family, serves as a calcium-dependent glycoprotein crucial for mediating cell adhesion and orchestrating various morphogenetic events in development. The encoded preproprotein undergoes processing to generate a mature functional protein. In mice, the absence of VE-Cadherin leads to in utero mortality, primarily attributed to vascular insufficiency resulting from heightened endothelial apoptosis. Notably, multiple distinct genes within the cadherin family, including this gene, are situated on chromosome 8. The expression profile reveals bias towards certain tissues, with prominent levels observed in the lung and subcutaneous fat pad among others, underscoring its importance in tissue-specific functions.

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 7.704 μg/mL, corresponding to a specific activity is 1.298×10² units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 7.704 μg/mL, corresponding to a specific activity is 1.298×10² units/mg.

Mouse

HEK293

C-10*His

P55284/NP_033998.2 (G25-A592)

12562  [NCBI]

Partial

GPNFPQIDTPNMLPAHHRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEFAGKIFGVDANTGNVLAYERLDREKVSEYFLTALIVDKNTNKNLEQPSSFTVKVHDINDNWPVFSHQVFNASVPEMSAIGTSVIRVTAVDADDPTVAGHATVLYQIVKGNEYFSIDNSGLIFTKIKNLDREKQAEYKIVVETQDALGLRGESGTATVMIRLEDINDNFPVFTQSTYTFSVPEDIRVGKPLGFLTVVDPDEPQNRMTKYSIMQGEYRDTFTIETDPKRNEGIIKPTKSLDYEVIQQYTFYIEATDPTIRYEYLSSTSGKNKAMVTINVLDVDEPPVFQRHFYHFKLPENQKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQFFRITKQGNIYNEKELDRETYAWYNLTVEANELDSRGNPVGKESIVQVYIEVLDENDNPPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNPKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHAKFHYLPVLISDNGVPSLTGTSTLTVGVCKCNEQGEFTFCEEMAA

approximately 85 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.