1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. VEGF & VEGFR Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. VEGFR
  5. VEGFR-1
  6. VEGFR-1 Protein, Mouse (HEK293, His)

VEGFR-1 Protein, Mouse (HEK293, His)

Cat. No.: HY-P73553
COA Handling Instructions

The VEGFR-1 protein is a key tyrosine protein kinase receptor. VEGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VEGFR-1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of VEGFR-1 Protein, Mouse (HEK293, His) is 737 a.a., with molecular weight of ~130 kDa.

For research use only. We do not sell to patients.

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Description

The VEGFR-1 protein is a key tyrosine protein kinase receptor. VEGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived VEGFR-1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of VEGFR-1 Protein, Mouse (HEK293, His) is 737 a.a., with molecular weight of ~130 kDa.

Background

VEGFR-1, a tyrosine-protein kinase, acts as a pivotal cell-surface receptor for VEGFA, VEGFB, and PGF, playing indispensable roles in embryonic vasculature development, angiogenesis regulation, cell survival, migration, macrophage function, chemotaxis, and cancer cell invasion. It functions as a positive regulator in the regression of postnatal retinal hyaloid vessels. While potentially acting as a negative regulator of embryonic angiogenesis by curbing excessive endothelial cell proliferation, VEGFR-1 also exhibits cell-type-specific promotion of proliferation, survival, and angiogenesis in adulthood, especially in response to PGF. With a high affinity for VEGFA, it modulates KDR signaling through heterodimer formation. Acting as a potential negative regulator of VEGFA signaling, it limits free VEGFA, hindering its binding to KDR. Ligand binding triggers various signaling cascades, including PLCG activation, leading to diacylglycerol and inositol 1,4,5-trisphosphate production, as well as protein kinase C activation. Additionally, VEGFR-1 mediates the phosphorylation of PIK3R1, initiating the phosphatidylinositol 3-kinase pathway, and activates MAPK1/ERK2, MAPK3/ERK1, and AKT1 signaling pathways. It also phosphorylates SRC, YES1, PLCG, and potentially CBL, facilitating the phosphorylation of AKT1 and PTK2/FAK1.

Species

Mouse

Source

HEK293

Tag

C-His;C-10*His

Accession

P35969/NP_034358.2 (Y23-E759)

Gene ID
Molecular Construction
N-term
VEGFR-1 (Y23-E759)
Accession # P35969/NP_034358.2
10*His
C-term
Synonyms
Vascular endothelial growth factor receptor 1; FLT; FLT1; FRT; VEGFR-1
AA Sequence

YGSGSKLKVPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKRLSITPPSACGRDNRQFCSTLTLDTAQANHTGLYTCRYLPTSTSKKKKAESSIYIFVSDAGSPFIEMHTDIPKLVHMTEGRQLIIPCRVTSPNVTVTLKKFPFDTLTPDGQRITWDSRRGFIIANATYKEIGLLNCEATVNGHLYQTNYLTHRQTNTILDVQIRPPSPVRLLHGQTLVLNCTATTELNTRVQMSWNYPGKATKRASIRQRIDRSHSHNNVFHSVLKINNVESRDKGLYTCRVKSGSSFQSFNTSVHVYEKGFISVKHRKQPVQETTAGRRSYRLSMKVKAFPSPEIVWLKDGSPATLKSARYLVHGYSLIIKDVTTEDAGDYTILLGIKQSRLFKNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPRPTITWLWHPCHHNHSKERYDFCTENEESFILDPSSNLGNRIESISQRMTVIEGTNKTVSTLVVADSQTPGIYSCRAFNKIGTVERNIKFYVTDVPNGFHVSLEKMPAEGEDLKLSCVVNKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEDILRKTEVLVRDSEAPHLLQNLSDYEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGAVESAAYLTVQGTSDKSNLE

Molecular Weight

Approximately 130 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
VEGFR-1 Protein, Mouse (HEK293, His)
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HY-P73553
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