VEGFR-1 Protein, Rat (HEK293, His)

VEGFR-1 protein is a tyrosine protein kinase receptor for VEGFA, VEGFB, and PGF and is critical for embryonic vasculature development, angiogenesis, cell survival, migration, macrophage function, chemotaxis, and cancer invasion. It actively regulates postnatal retinal vitreous vascular degeneration and may act as a negative regulator of embryonic angiogenesis. VEGFR-1 Protein, Rat (HEK293, His) is the recombinant rat-derived VEGFR-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of VEGFR-1 Protein, Rat (HEK293, His) is 736 a.a., with molecular weight of ~110-130 kDa.

VEGFR-1 protein, a tyrosine-protein kinase, serves as a cell-surface receptor for VEGFA, VEGFB, and PGF, playing a crucial role in embryonic vasculature development, angiogenesis regulation, cell survival, migration, macrophage function, chemotaxis, and cancer cell invasion. It acts as a positive regulator of postnatal retinal hyaloid vessel regression and may function as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. In adulthood, VEGFR-1 can promote endothelial cell proliferation, survival, and angiogenesis, with its function in cell proliferation being cell-type specific. It facilitates PGF-mediated proliferation of endothelial cells and certain cancer cell types but does not promote proliferation of normal fibroblasts. With a high affinity for VEGFA and relatively low protein kinase activity, VEGFR-1 may function as a negative regulator of VEGFA signaling by limiting free VEGFA and preventing its binding to KDR. It modulates KDR signaling by forming heterodimers with KDR. Ligand binding activates multiple signaling cascades, including PLCG-mediated diacylglycerol and inositol 1,4,5-trisphosphate production, protein kinase C activation, phosphorylation of PIK3R1, leading to phosphatidylinositol 3-kinase activation, and subsequent downstream signaling pathways. VEGFR-1 also mediates the activation of MAP kinase and AKT1 signaling pathways, phosphorylates SRC, YES1, PLCG, and potentially CBL, and promotes phosphorylation of AKT1 and PTK2/FAK1, highlighting its diverse and integral role in regulating complex cellular processes.

Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 0.1 μg/mL(100 μL/well) can bind biotinylated Rat VEGFR-1. The ED₅₀ for this effect is 76.55 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 0.1 μg/mL (100 μL/well) can bind biotinylated Rat VEGFR-1. The ED₅₀ for this effect is 76.55 ng/mL.

Rat

HEK293

C-His

P53767 (Y23-E758)

54251  [NCBI]

YCSGSKLKGPELSLKGTQHVMQAGQTLFLKCRGEAAHSWSLPTTVSQEDKKLSVTRSACGRNNRQFCSTLTLNMAQANHTGLYSCRYLPKSTSKEKKMESAIYIFVSDAGSPFIEMHSDIPKLVHMTEGRELIIPCRVTSPNITVTLKKFPFDALTPDGQRIAWDSRRGFIIANATYKEIGLLTCEATVNGHLYQTSYLTHRQTNTILDVQISPPSPVRFLRGQTLVLNCTVTTDLNTRVQMSWNYPGKATKRASIRQRIDQSNPHSNVFHSVLKINNVESRDKGLYTCRVKSGSSFRTFNTSVHVYEKGFISVKHRKQQVQETIAGKRSHRLSMKVKAFPSPEVVWLKDGVPATEKSARYSVHGYSLIIKDVTAEDAGDYTILLGIKQSKLFRNLTATLIVNVKPQIYEKSVSSLPSPPLYPLGSRQVLTCTVYGIPQPTIKWLWHPCHYNHSKERNDFCFGSEESFILDSSSNIGNRIEGITQRMMVIEGTNKTVSTLVVADSRTPGSYSCKAFNKIGTVERDIRFYVTDVPNGFHVSLEKIPTEGEDLKLSCVVSKFLYRDITWILLRTVNNRTMHHSISKQKMATTQDYSITLNLVIKNVSLEDSGTYACRARNIYTGEEILRKTEVLVRDLEAPLLLQNLSDHEVSISGSTTLDCQARGVPAPQITWFKNNHKIQQEPGIILGPGNSTLFIERVTEEDEGVYRCRATNQKGVVESSAYLTVQGTSDKSNLE

Approximately 110-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.