Copy Link Print Information

VTI1A Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

VTI1A protein is a V-SNARE that mediates vesicle trafficking and promotes lipid bilayer fusion by interacting with t-SNARE on target membranes. It plays a role in vesicle trafficking from late endosomes to the trans-Golgi network. VTI1A Protein, Human (HEK293, His) is the recombinant human-derived VTI1A protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • VTI1A Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

VTI1A protein is a V-SNARE that mediates vesicle trafficking and promotes lipid bilayer fusion by interacting with t-SNARE on target membranes. It plays a role in vesicle trafficking from late endosomes to the trans-Golgi network. VTI1A Protein, Human (HEK293, His) is the recombinant human-derived VTI1A protein, expressed by HEK293 , with N-His labeled tag.

Background

VTI1A, a vesicle-associated soluble N-ethylmaleimide-sensitive factor attachment protein receptor (V-SNARE), plays a crucial role in mediating vesicle transport pathways through interactions with target membrane t-SNAREs, contributing to the specificity of vesicle trafficking and facilitating lipid bilayer fusion. This protein is implicated in vesicular transport from late endosomes to the trans-Golgi network and participates, along with VAMP7, in a non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. Additionally, VTI1A may be associated with increased cytokine secretion observed during cellular senescence. It interacts with distinct SNARE complexes containing either STX5 or STX6, and forms a complex with NAPA and, to a lesser extent, with NAPG. Furthermore, VTI1A is identified in a complex alongside STX6, STX12, VAMP4, and itself, highlighting its role in diverse intracellular trafficking processes.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His
  • Accession

    Q96AJ9-2 (M1-R187)

  • Gene ID
    143187  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • VTI1A (M1-R187)
      Accession # Q96AJ9-2
    • C-term
  • Protein Length

    Partial

  • Synonyms

    VTI1A; Vesicle Transport Through Interaction With T-SNAREs 1A; Vti1-Rp2; Vesicle Transport Through Interaction With T-SNAREs Homolog 1A; Vesicle Transport V-SNARE Protein Vti1-Like 2; MVti1; Vesicle Transport Through Interaction With T-SNAREs Homolog 1A (

  • AA Sequence

    MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEAKELLEQMDLEVREIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDDGNSSENQRAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDREKIQRARERLRETDANLGKSSRILTGMLRR

  • Predicted Molecular Mass

    21.8 kDa

  • Molecular Weight

    Approximately 29 kDa & 25 kDa & 20 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • VTI1A Protein, Human (HEK293, His) Product Image
    1/16
  • VTI1A Protein, Human (HEK293, His) Product Image