VTI1A Protein, Human (HEK293, His)

VTI1A protein is a V-SNARE that mediates vesicle trafficking and promotes lipid bilayer fusion by interacting with t-SNARE on target membranes. It plays a role in vesicle trafficking from late endosomes to the trans-Golgi network. VTI1A Protein, Human (HEK293, His) is the recombinant human-derived VTI1A protein, expressed by HEK293 , with N-His labeled tag.

VTI1A, a vesicle-associated soluble N-ethylmaleimide-sensitive factor attachment protein receptor (V-SNARE), plays a crucial role in mediating vesicle transport pathways through interactions with target membrane t-SNAREs, contributing to the specificity of vesicle trafficking and facilitating lipid bilayer fusion. This protein is implicated in vesicular transport from late endosomes to the trans-Golgi network and participates, along with VAMP7, in a non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. Additionally, VTI1A may be associated with increased cytokine secretion observed during cellular senescence. It interacts with distinct SNARE complexes containing either STX5 or STX6, and forms a complex with NAPA and, to a lesser extent, with NAPG. Furthermore, VTI1A is identified in a complex alongside STX6, STX12, VAMP4, and itself, highlighting its role in diverse intracellular trafficking processes.

Human

HEK293

N-His

Q96AJ9-2 (M1-R187)

143187  [NCBI]

Partial

MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEAKELLEQMDLEVREIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDDGNSSENQRAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDREKIQRARERLRETDANLGKSSRILTGMLRR

Approximately 29 kDa & 25 kDa & 20 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.