YES1 Protein, Human (sf9, His-GST)

YES1 is a non-receptor protein tyrosine kinase that regulates processes such as cell growth, survival, apoptosis, and cell-cell adhesion. It is activated by RTKs (EGFR, PDGFR, CSF1R, FGFR) to phosphorylate downstream substrates. YES1 Protein, Human (sf9, His-GST) is the recombinant human-derived YES1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of YES1 Protein, Human (sf9, His-GST) is 542 a.a., with molecular weight of ~75 kDa.

YES1, a non-receptor protein tyrosine kinase, is a pivotal regulator of diverse cellular processes, including cell growth, survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Activation by various receptor tyrosine kinases (RTKs), such as EGFR, PDGFR, CSF1R, and FGFR, results in its recruitment to phosphorylated receptors, initiating downstream substrate activation and phosphorylation. Upon EGFR activation, YES1 facilitates the phosphorylation of PARD3, promoting epithelial tight junction assembly. At cell-cell contacts, it stimulates the FYN and FER tyrosine kinases, leading to the phosphorylation of specific junctional components like CTNND1. In response to T-cell stimulation by CXCL12, YES1 phosphorylates collapsin response mediator protein 2/DPYSL2, inducing T-cell migration. Additionally, it participates in CD95L/FASLG signaling, mediating AKT-dependent cell migration. YES1's involvement in cell cycle progression is evident through the phosphorylation of cyclin-dependent kinase 4/CDK4, regulating the G1 phase, and its role in G2/M progression and cytokinesis. Furthermore, YES1 catalyzes the phosphorylation of the organic cation transporter OCT2, enhancing its transport activity.

The specific activity was determined to be > 35 nmol/min/mg using Poly (Glu,Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P07947 (G2-L543)

7525  [NCBI]

Approximately 75 kDa

Greater than 80% as determined by reducing SDS-PAGE

Solution.

Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% gly, 0.5 mM TCEP, pH 8.0

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.