YES1 Protein, Human (Active, sf9, His-GST)

YES1 is a non-receptor protein tyrosine kinase that regulates processes such as cell growth, survival, apoptosis, and cell-cell adhesion. It is activated by RTKs (EGFR, PDGFR, CSF1R, FGFR) to phosphorylate downstream substrates. YES1 Protein, Human (sf9, His-GST) is the recombinant human-derived YES1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

YES1, a non-receptor protein tyrosine kinase, is a pivotal regulator of diverse cellular processes, including cell growth, survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Activation by various receptor tyrosine kinases (RTKs), such as EGFR, PDGFR, CSF1R, and FGFR, results in its recruitment to phosphorylated receptors, initiating downstream substrate activation and phosphorylation. Upon EGFR activation, YES1 facilitates the phosphorylation of PARD3, promoting epithelial tight junction assembly. At cell-cell contacts, it stimulates the FYN and FER tyrosine kinases, leading to the phosphorylation of specific junctional components like CTNND1. In response to T-cell stimulation by CXCL12, YES1 phosphorylates collapsin response mediator protein 2/DPYSL2, inducing T-cell migration. Additionally, it participates in CD95L/FASLG signaling, mediating AKT-dependent cell migration. YES1's involvement in cell cycle progression is evident through the phosphorylation of cyclin-dependent kinase 4/CDK4, regulating the G1 phase, and its role in G2/M progression and cytokinesis. Furthermore, YES1 catalyzes the phosphorylation of the organic cation transporter OCT2, enhancing its transport activity.

The specific activity was determined to be ≥ 35 nmol/min/mg using Poly (Glu,Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P07947 (G2-L543)

7525  [NCBI]

Full Length of Mature Protein

GCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL

Approximately 75 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 80%, as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25% glycerol, 0.5 mM GSH, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.