ZYX Protein, Human (HEK293, His)

ZYX protein is a focal adhesion protein that binds α-actin and CRP to target TES and ENA/VASP to focal adhesions to form actin-rich structures. It may play a crucial role in adhesion-stimulated gene expression. ZYX Protein, Human (HEK293, His) is the recombinant human-derived ZYX protein, expressed by HEK293 , with N-6*His labeled tag. The total length of ZYX Protein, Human (HEK293, His) is 571 a.a., with molecular weight of ~65.1 kDa.

The ZYX protein functions as an adhesion plaque protein with a pivotal role in binding alpha-actinin and the CRP protein. Its significance lies in targeting TES and ENA/VASP family members to focal adhesions and contributing to the formation of actin-rich structures. ZYX may be a crucial component of a signal transduction pathway that orchestrates adhesion-stimulated changes in gene expression. Notably, it interacts with the HPV type 6 protein E6, while showing limited interaction with E6 proteins from HPV types 11, 16, or 18. The Pro-rich regions of ZYX engage in interactions with the EVH1 domains of ENAH, EVL, and VASP, and it also interacts with the first LIM domain of TES, NEBL (isoform 2), and SYNPO2, underscoring its diverse molecular associations and its role in mediating cellular signaling events.

Human

HEK293

N-6*His

Q15942 (A2-T572)

7791  [NCBI]

AAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Approximately 65.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.