spectrophotometric substrate

By Targets:	Biochemical Assay Reagents (10) Fluorescent Dye (2) HCV Protease (1) Isotope-Labeled Compounds (1) Leukotriene Receptor (1)
By Research Areas:	Cancer (1) Infection (1) Metabolic Disease (1)

Targets Recommended: MARCKS

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-15928

4-Nitrophenyl phosphate disodium 1 Publications Verification p-Nitrophenyl phosphate disodium	Fluorescent Dye	Others
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-15926

ONPG Maximum Cited Publications 6 Publications Verification 2-Nitrophenyl β-D-galactopyranoside	Biochemical Assay Reagents	Others
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.

HY-15927

4-Nitrophenyl β-D-glucopyranoside 1 Publications Verification	Biochemical Assay Reagents	Metabolic Disease
4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis .

HY-W129633

2,6-Dichlorophenolindophenol sodium hydrate 1 Publications Verification DCIP sodium hydrate; DPIP sodium hydrate	Biochemical Assay Reagents	Others
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae .

HY-W045537

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium	Biochemical Assay Reagents	Others
Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.

HY-P4522A

Bz-Ala-Arg TFA	Biochemical Assay Reagents	Others
Bz-Ala-Arg TFA is a dipeptide. Bz-Ala-Arg TFA is also a substrate of human pancreatic carboxypeptidase B and plasma carboxypeptidase N .

HY-P4522

Bz-Ala-Arg	Biochemical Assay Reagents	Others
Bz-Ala-Arg is a dipeptide. Bz-Ala-Arg is also a substrate of human pancreatic carboxypeptidase B and plasma carboxypeptidase N .

HY-130767A

Naphthol AS-TR phosphate disodium	Biochemical Assay Reagents	Cancer
Naphthol AS-TR Phosphate disodium is a water-soluble dye commonly used as an enzymatic substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate disodium has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase, forming a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

HY-15926S

ONPG-13C 2-Nitrophenyl β-D-galactopyranoside-¹³C	Isotope-Labeled Compounds	Others
ONPG- ¹³C is the ¹³C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit .

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

HY-158735

Thioarginine hydrobromide	Biochemical Assay Reagents	Others
Thioarginine hydrobromide is a colorimetric substrate for arginase that can be used in the spectrophotometric assay of arginase .

HY-116022B

4-Nitrophenyl phosphate ditromethamine p-Nitrophenyl phosphate ditromethamine	Biochemical Assay Reagents	Others
4-Nitrophenyl phosphate ditromethamine is an enzyme substrate commonly used in biochemical assays to measure the activity of various phosphatases, including alkaline phosphatase. 4-Nitrophenylphosphate has unique chemical properties that allow it to be hydrolyzed by phosphatases, releasing a yellow product that can be detected spectrophotometrically.

HY-138241

PBD-BODIPY	Fluorescent Dye	Others
PBD-BODIPY is a probe for the spectrophotometric measurement of autoxidation reactions. Co-autoxidation of the PBD-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring loss of absorbance at 591 nm. PBD-BODIPY has been used to measure the activity of radical-trapping antioxidants in cell-free assays. It has also been used as a fluorescent probe for the detection of epoxidation activity.

HY-130767

Naphthol AS-TR phosphate	Biochemical Assay Reagents	Others
Naphthol AS-TR Phosphate is a water-soluble dye commonly used as an enzyme substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase to form a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

HY-W612175

H-Ala-pNA	Leukotriene Receptor	Others
H-Ala-pNA is an L-amino acid p-nitroaniline (pNA) derivative and a specific substrate for leukotriene A4 hydrolase. The D-enantiomer of H-Ala-pNA shows no activity toward leukotriene A4 hydrolase. H-Ala-pNA can be catalytically hydrolyzed by leukotriene A4 hydrolase, and the p-nitroaniline produced during the reaction is monitored spectrophotometrically at 405 nm to enable quantitative detection of enzyme activity. H-Ala-pNA is used to evaluate the potency of inhibitors targeting the amidase activity of leukotriene A4 hydrolase .

Cat. No.	Product Name	Type

HY-15928

4-Nitrophenyl phosphate disodium 1 Publications Verification p-Nitrophenyl phosphate disodium	Fluorescent Dyes
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

HY-P4039

Ac-EEVVAC-pNA	Fluorescent Dyes
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

Cat. No.	Product Name	Type

HY-15926

ONPG Maximum Cited Publications 6 Publications Verification 2-Nitrophenyl β-D-galactopyranoside	Biochemical Assay Reagents
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.

HY-15927

4-Nitrophenyl β-D-glucopyranoside 1 Publications Verification	Biochemical Assay Reagents
4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis .

4-Nitrophenyl β-D-glucopyranoside

1 Publications Verification

Biochemical Assay Reagents

4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis .

HY-W129633

2,6-Dichlorophenolindophenol sodium hydrate 1 Publications Verification DCIP sodium hydrate; DPIP sodium hydrate	Biochemical Assay Reagents
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae .

2,6-Dichlorophenolindophenol sodium hydrate

1 Publications Verification

DCIP sodium hydrate; DPIP sodium hydrate

Biochemical Assay Reagents

2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae .

HY-W045537

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium	Biochemical Assay Reagents
Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.

5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium

Biochemical Assay Reagents

Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate is commonly used as a substrate for phosphatase assays in biochemistry and molecular biology. This compound is converted by phosphatases to a yellow compound that can be easily measured spectrophotometrically or fluorometrically. Furthermore, it has been used as a useful reagent in enzyme-catalyzed reactions and a tool for studying signal transduction pathways.

HY-116022B

4-Nitrophenyl phosphate ditromethamine p-Nitrophenyl phosphate ditromethamine	Biochemical Assay Reagents
4-Nitrophenyl phosphate ditromethamine is an enzyme substrate commonly used in biochemical assays to measure the activity of various phosphatases, including alkaline phosphatase. 4-Nitrophenylphosphate has unique chemical properties that allow it to be hydrolyzed by phosphatases, releasing a yellow product that can be detected spectrophotometrically.

HY-130767

Naphthol AS-TR phosphate	Biochemical Assay Reagents
Naphthol AS-TR Phosphate is a water-soluble dye commonly used as an enzyme substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase to form a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

Naphthol AS-TR phosphate

Biochemical Assay Reagents

Naphthol AS-TR Phosphate is a water-soluble dye commonly used as an enzyme substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase to form a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

Cat. No.	Product Name	Target	Research Area

HY-P2573

Suc-AAPF-pNA 2 Publications Verification Suc-Ala-Ala-Pro-Phe-pNA	Peptides	Others
Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the K_m of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils .

HY-P4522A

Bz-Ala-Arg TFA	Biochemical Assay Reagents	Others
Bz-Ala-Arg TFA is a dipeptide. Bz-Ala-Arg TFA is also a substrate of human pancreatic carboxypeptidase B and plasma carboxypeptidase N .

HY-P4522

Bz-Ala-Arg	Biochemical Assay Reagents	Others
Bz-Ala-Arg is a dipeptide. Bz-Ala-Arg is also a substrate of human pancreatic carboxypeptidase B and plasma carboxypeptidase N .

HY-P4039

Ac-EEVVAC-pNA	HCV Protease	Infection
Ac-EEVVAC-pNA is a chromogenic substrate for a continuous spectrophotometric assay of HCV NS3 protease. The sequence EEVVAC is derived from the 5A-5B cleavage junction of the HCV polyprotein .

Cat. No.	Product Name	Chemical Structure

HY-15926S

ONPG-13C
ONPG- ¹³C is the ¹³C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit .

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
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Fluorescent Dyes

Biochemical Assay Reagents

Peptides

Isotope-Labeled Compounds