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  4. Naphthol AS-TR phosphate disodium

Naphthol AS-TR phosphate disodium 

Cat. No.: HY-130767A Purity: 99.89%
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Naphthol AS-TR Phosphate disodium is a water-soluble dye commonly used as an enzymatic substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate disodium has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase, forming a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

For research use only. We do not sell to patients.

Naphthol AS-TR phosphate disodium Chemical Structure

Naphthol AS-TR phosphate disodium Chemical Structure

CAS No. : 4264-93-1

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Description

Naphthol AS-TR Phosphate disodium is a water-soluble dye commonly used as an enzymatic substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate disodium has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase, forming a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.

Molecular Weight

435.71

Formula

C18H13ClNNa2O5P
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=C(OP(O[Na])(O[Na])=O)C=C2C=CC=CC2=C1)NC3=CC=C(Cl)C=C3C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (229.51 mM; Need ultrasonic)

DMSO : 50 mg/mL (114.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2951 mL 11.4755 mL 22.9510 mL
5 mM 0.4590 mL 2.2951 mL 4.5902 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.2951 mL 11.4755 mL 22.9510 mL 57.3776 mL
5 mM 0.4590 mL 2.2951 mL 4.5902 mL 11.4755 mL
10 mM 0.2295 mL 1.1476 mL 2.2951 mL 5.7378 mL
15 mM 0.1530 mL 0.7650 mL 1.5301 mL 3.8252 mL
20 mM 0.1148 mL 0.5738 mL 1.1476 mL 2.8689 mL
25 mM 0.0918 mL 0.4590 mL 0.9180 mL 2.2951 mL
30 mM 0.0765 mL 0.3825 mL 0.7650 mL 1.9126 mL
40 mM 0.0574 mL 0.2869 mL 0.5738 mL 1.4344 mL
50 mM 0.0459 mL 0.2295 mL 0.4590 mL 1.1476 mL
60 mM 0.0383 mL 0.1913 mL 0.3825 mL 0.9563 mL
80 mM 0.0287 mL 0.1434 mL 0.2869 mL 0.7172 mL
100 mM 0.0230 mL 0.1148 mL 0.2295 mL 0.5738 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Naphthol AS-TR phosphate disodium Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naphthol AS-TR phosphate disodium4264-93-1Biochemical Assay ReagentsInhibitorinhibitorinhibit

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