Naphthol AS-TR phosphate disodium
Based on 1 Customer Validation
Naphthol AS-TR Phosphate disodium is a water-soluble dye commonly used as an enzymatic substrate in various biochemical assays to detect alkaline phosphatase activity. Naphthol AS-TR Phosphate disodium has unique chemical properties that allow it to be hydrolyzed by alkaline phosphatase, forming a colored product that can be detected spectrophotometrically. This makes it a useful tool for monitoring enzyme activity in biological samples such as serum or urine.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 4264-93-1
- Formula: C18H13ClNNa2O5P
- Molecular Weight:435.71
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 4264-93-1
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Appearance Solid
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Molecular Weight 435.71
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Formula C18H13ClNNa2O5P
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Color Off-white to light yellow
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SMILES
O=C(C1=C(OP(O[Na])(O[Na])=O)C=C2C=CC=CC2=C1)NC3=CC=C(Cl)C=C3C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 100 mg/mL (229.51 mM; Need ultrasonic)
DMSO : 50 mg/mL (114.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.2951 mL | 11.4755 mL | 22.9510 mL | 57.3776 mL |
| 5 mM | 0.4590 mL | 2.2951 mL | 4.5902 mL | 11.4755 mL | |
| 10 mM | 0.2295 mL | 1.1476 mL | 2.2951 mL | 5.7378 mL | |
| 15 mM | 0.1530 mL | 0.7650 mL | 1.5301 mL | 3.8252 mL | |
| 20 mM | 0.1148 mL | 0.5738 mL | 1.1476 mL | 2.8689 mL | |
| 25 mM | 0.0918 mL | 0.4590 mL | 0.9180 mL | 2.2951 mL | |
| 30 mM | 0.0765 mL | 0.3825 mL | 0.7650 mL | 1.9126 mL | |
| 40 mM | 0.0574 mL | 0.2869 mL | 0.5738 mL | 1.4344 mL | |
| 50 mM | 0.0459 mL | 0.2295 mL | 0.4590 mL | 1.1476 mL | |
| 60 mM | 0.0383 mL | 0.1913 mL | 0.3825 mL | 0.9563 mL | |
| 80 mM | 0.0287 mL | 0.1434 mL | 0.2869 mL | 0.7172 mL | |
| 100 mM | 0.0230 mL | 0.1148 mL | 0.2295 mL | 0.5738 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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